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Nucleotide sequence of tomato ringspot virus RNA-2.
The sequence of tomato ringspot virus (TomRSV) RNA-2 has been determined. It is 7273 nucleotides in length excluding the 3' poly(A) tail and contains a single long open reading frame (ORF) of 5646Expand
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Detection and Partial Characterization of a Second Closterovirus Associated with Little Cherry Disease, Little cherry virus-2.
ABSTRACT Little cherry disease (LChD) is a complex and serious viral disease of cherry. Although originally described almost 70 years ago, there has been little progress in identifying the causalExpand
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Nucleotide sequence of tomato ringspot virus RNA1.
The nucleotide sequence of tomato ringspot nepovirus (TomRSV) RNA1 has been determined. TomRSV RNA1 is 8214 nucleotides in length, excluding the 3' poly(A) tail, and contains a single long openExpand
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Transatlantic Trade and Investment Partnership (TTIP)
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Range of phytoplasma concentrations in various plant hosts as determined by competitive polymerase chain reaction.
ABSTRACT For competitive polymerase chain reaction (PCR), an internal standard DNA template was developed that consisted of a highly conserved, internally deleted 16S rDNA fragment of an asterExpand
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Comparison of the 5′ and 3′ termini of tomato ringspot virus RNA1 and RNA2: Evidence for RNA recombination☆
Abstract The sequences of the 5′ terminal 1140 and 3′ terminal 1546 nt of tomato ringspot virus (TomRSV) RNA1 have been determined. These sequences share a high degree of nucleotide sequenceExpand
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Application of Next Generation Sequencing for Diagnostic Testing of Tree Fruit Viruses and Viroids.
Conventional detection of viruses and virus-like diseases of plants is accomplished using a combination of molecular, serological, and biological indexing. These are the primary tools used by plantExpand
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Discovery of Negative-Sense RNA Viruses in Trees Infected with Apple Rubbery Wood Disease by Next-Generation Sequencing.
Apple rubbery wood is a disease of apple found around the world, often associated with Apple flat limb disease, and regulated in many countries. Despite its long history in apple cultivation, theExpand
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Epidemiology and Genetic Diversity of Grapevine Leafroll-Associated Viruses in British Columbia.
Grapevine leafroll disease (GLD) is a complex associated with one or more virus species belonging to the family Closteroviridae. The majority of viruses in this complex are vectored by one or moreExpand
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Screening for plant viruses by next generation sequencing using a modified double strand RNA extraction protocol with an internal amplification control.
The majority of plant viruses contain RNA genomes. Detection of viral RNA genomes in infected plant material by next generation sequencing (NGS) is possible through the extraction and sequencing ofExpand
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