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BRENDA in 2013: integrated reactions, kinetic data, enzyme function data, improved disease classification: new options and contents in BRENDA
The integration of the EnzymeDetector offers an automatic comparison, evaluation and prediction of enzyme function annotations for prokaryotic genomes.
BRENDA, the enzyme information system in 2011
The BRENDA enzyme information system is the main collection of enzyme functional and property data for the scientific community and now provides new viewing options such as the display of the statistics of functional parameters and the 3D view of protein sequence and structure features.
Anaerobic growth of Methanosarcina acetivorans C2A on carbon monoxide: an unusual way of life for a methanogenic archaeon.
  • M. Rother, W. Metcalf
  • Chemistry, Medicine
    Proceedings of the National Academy of Sciences…
  • 30 November 2004
Interestingly, acetate and formate were the major metabolic end products as shown by 13C NMR studies and enzymatic analysis of culture supernatants, strongly suggesting that M. acetivorans conserves energy by means of this acetogenic and formigenic process.
Carbon monoxide-dependent energy metabolism in anaerobic bacteria and archaea
Recent insights into the CO-dependent physiology of anaerobic microorganisms with a focus on methanogenic archaea are summarized and Methanosarcina acetivorans, thought to strictly rely on the process of methanogenesis, exemplifies how the beneficial redox properties of CO can be exploited in unexpected ways by anaerobia microorganisms.
Genetic and proteomic analyses of CO utilization by Methanosarcina acetivorans
Qualitative proteomic analyses confirm a recent report in showing that the bifunctional CODH/acetyl-CoA synthase (ACS) system, two enzymes involved in CO2-reduction, and a peculiar protein homologous to both corrinoid proteins and methyltransferases are synthesized at elevated levels in response to CO.
Selenoproteins in Archaea and Gram-positive bacteria.
Genome Copy Numbers and Gene Conversion in Methanogenic Archaea
It was shown that in the absence of selection very fast equalization of genomes in M. maripaludis took place probably via a gene conversion mechanism, and that the velocity of this phenomenon is inversely correlated to the strength of selection.
New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species.
A series of plasmid vectors that utilize the site-specific recombination system for construction of reporter gene fusions and for tetracycline regulated expression of cloned genes are reported.
Selenocysteine-Containing Proteins in Anaerobic Benzoate Metabolism of Desulfococcus multivorans
The results suggest that the dearomatization process in D. multivorans is not catalyzed by the ATP-dependent Fe-S enzyme benzoyl-CoA reductase as in facultative anaerobes but rather involves unknown molybdenum- and selenocysteine-containing proteins.