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Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells
It is shown that human fetal and adult fibroblast feeders support prolonged undifferentiated HES cell growth of existing cell lines and are superior to cell-free matrices supplemented with human or MEF feeder–conditioned medium. Expand
The Transcriptome Profile of Human Embryonic Stem Cells as Defined by SAGE
The overlapping similarities and differences in gene expression profiles of human and mouse ES cells provide a foundation for a detailed and concerted dissection of the molecular and cellular mechanisms governing their pluripotency, directed differentiation into specific cell types, and extended ability for self‐renewal. Expand
Comparative Evaluation of Various Human Feeders for Prolonged Undifferentiated Growth of Human Embryonic Stem Cells
It is reported that two adult skin fibroblast cell lines established in‐house from abdominal skin biopsies supported prolonged undifferentiated hES cell growth for over 30 weekly passages in culture. Expand
Comparative growth behaviour and characterization of stem cells from human Wharton's jelly.
Wharton's jelly stem cells are not as pluripotent as hESC but widely multipotent, and have the advantages of being able to be scaled up easily and not inducing teratomas. Expand
An Efficient and Safe Xeno‐Free Cryopreservation Method for the Storage of Human Embryonic Stem Cells
The cryopreservation technique described here should complement xeno‐free culture conditions for hESCs already in refinement and will prove very useful for the setting up of hESC banks throughout the world. Expand
History and perspective of stem cell research.
  • A. Bongso, M. Richards
  • Biology, Medicine
  • Best practice & research. Clinical obstetrics…
  • 1 December 2004
Stem cell research opens-up the new field of 'cell-based therapies' and, as such, several safety measures have also to be evaluated. Expand
Differential effect of solar light in increasing the toxicity of silver and titanium dioxide nanoparticles to a fish cell line and zebrafish embryos.
Observations that solar light could induce physicochemical transformation of TiO2 and Ag NPs and enhance their toxic potential emphasizes the need for conducting future toxicity studies under environmentally relevant exposure conditions to guide decision making on the safe handling of NPs. Expand
Comparative evaluation of different in vitro systems that stimulate germ cell differentiation in human embryonic stem cells.
POFs proved to be the best system for initiating germ cell differentiation, as shown by increases in the expression of several germ cell marker genes in EBs that were cocultured with POFs. Expand
Induced pluripotent stem cells: a new tool for toxicology screening?
In recent years, stem cells have generated much interest as a potential tool for pharmacological and toxicology screening (CIRM 2008; ABPI 2008), due to various shortcomings of currently utilizedExpand
Eml1 loss impairs apical progenitor spindle length and soma shape in the developing cerebral cortex
The HeCo mutant mouse exhibits subcortical band heterotopia (SBH), likely to be initiated by progenitor delamination from the VZ early during corticogenesis, and microtubule (MT)-associated protein of the EMAP family is impaired in these mice. Expand