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Genome sequence of the model mushroom Schizophyllum commune
TLDR
The wood-degrading fungus Schizophyllum commune is both a genetically tractable model for studying mushroom development and a likely source of enzymes capable of efficient degradation of lignocellulosic biomass.
Effect of cytochalasin A on apical growth, actin cytoskeleton organization and enzyme secretion in Aspergillus nidulans.
TLDR
Evidence is provided that a polymerized actin cytoskeleton is required for normal apical growth, hyphal tip shape and polarized enzyme secretion in A. nidulans.
T-DNA transfer and integration in the ectomycorrhizal fungus Suillus bovinus using hygromycin B as a selectable marker
TLDR
Dikaryotic mycelium of the ectomycorrhizal fungus Suillus bovinus was transformed with recombinant hygromycin B phosphotransferase and enhanced green fluorescent protein (EGFP) genes fused with a heterologous fungal promoter and CaMV 35S terminator to make possible the development of tagged mutagenesis and targeted gene disruption technology for S. Bovinus.
Genetic transformation of ectomycorrhizal fungi mediated by Agrobacterium tumefaciens
TLDR
Transforming ectomycorrhiza-forming basidiomycetes Suillus bovinus, Hebeloma cylindrosporum, and Paxillus involutus based on Agrobacterium tumefaciens -mediated T-DNA transfer found transformants from all three investigated species contained the GPDScP-Shble-GPDScT construct, although the fate of the foreign DNA could not be determined.
Multiple genes encoding pheromones and a pheromone receptor define the B beta 1 mating-type specificity in Schizophyllum commune.
TLDR
A comparison of the DNA sequences of the B alpha 1 and B beta 1 loci suggests that each arose from a common ancestral sequence, allowing us to speculate about the evolution of this unique series of regulatory genes.
Basidiomycete Mating Type Genes and Pheromone Signaling
ABSTRACT The genome sequences of the basidiomycete Agaricomycetes species Coprinopsis cinerea, Laccaria bicolor, Schizophyllum commune, Phanerochaete chrysosporium, and Postia placenta, as well as of
Movement of generative cell and vegetative nucleus in tobacco pollen tubes is dependent on microtubule cytoskeleton but independent of the synthesis of callose plugs
TLDR
In oryzalin tubes with several callose plugs, the VN and GC could be in or near the grain, indicating that callose plug synthesis is not dependent on the movement of Vn and GC into the tube.
Comparative immunocytochemistry of the cytoskeleton in filamentous fungi with dikaryotic and multinucieate hyphae
TLDR
Indirect immunofluorescence microscopy of microtubules of five filamentous fungi and four homobasidiomycetes indicated that the gross structure of the microtubule cytoskeleton was comparable in spite of the multinucleate and dikaryotic cell conditions.
Transcriptome and Functional Analysis of Mating in the Basidiomycete Schizophyllum commune
TLDR
A functional characterization and transcriptome analysis that enabled a comprehensive study of the mating type loci of the mushroom Schizophyllum commune indicated a role of receptor-pheromone interaction in clamp fusion, and did not support hypotheses envisioning a receptor localization to the nuclear membrane facilitating recognition between the two different nuclei present in each dikaryotic cell.
Detection of extracellular cellulolytic and proteolytic activity in ectomycorrhizal fungi and Heterobasidion annosum (Fr.) Bref.
TLDR
Proteolytic and cellulolytic activities were studied in the ectomycorrhizal fungi Amanita regalis, Paxillus involutus, Suillus bovinus and the necrotrophic root pathogenic fungus Heterobasidion annosum using plate assays, native gel electrophoresis and substrate gel replicas.
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