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Electro-spinning of pure collagen nano-fibres - just an expensive way to make gelatin?
TLDR
Electro-spinning of collagen out of fluoroalcohols effectively denatures this biopolymer, and thus appears to defeat its purpose, namely to create biomimetic scaffolds emulating the collagen structure and function of the extracellular matrix. Expand
Concise Review: Multifaceted Characterization of Human Mesenchymal Stem Cells for Use in Regenerative Medicine
TLDR
It is proposed that cell surface marker profiles, bone‐forming capacities in ectopic and orthotopic models, and other functionalities of MSCs should be characterized prior to use in clinical applications as part of comprehensive and uniform guidelines and release criteria for their clinical‐grade production to achieve predictably favorable treatment outcomes for stem cell therapy. Expand
Self-healing collodion baby: a dynamic phenotype explained by a particular transglutaminase-1 mutation.
TLDR
Molecular modeling and biochemical assays of mutant proteins under elevated hydrostatic pressure suggest significantly reduced activity in G278R and a chelation of water molecules in D490G that locks the mutated enzyme in an inactive trans conformation in utero. Expand
Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect
TLDR
Implementing EVE in fibroblast culture accelerated conversion of procollagen to collagen by Procollagen C‐proteinase and proteolytic modification of its allosteric regulator, PCOLCE1 led to a 20–30‐ and 3–6‐fold increased collagen deposition in two‐ and three‐dimensional cultures, respectively. Expand
SPINK5 and Netherton syndrome: novel mutations, demonstration of missing LEKTI, and differential expression of transglutaminases.
TLDR
These studies demonstrate LEKTI deficiency in the epidermis and in hair roots at the protein level and an aberrant expression of other proteins, especially transglutaminase1 and 3, which may account for the impaired epidermal barrier in NTS. Expand
Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies.
TLDR
Alternative strategies that enable cells to re-create their own microenvironment in vitro, using carbohydrate-based macromolecules as culture media additives that create an excluded volume effect at defined fraction volume occupancies are discussed. Expand
Not all MSCs can act as pericytes: functional in vitro assays to distinguish pericytes from other mesenchymal stem cells in angiogenesis.
TLDR
Functional data suggest that pericytes represent a subpopulation of MSCs in bm with a specialized role in vascular biology, however, these functions are not inherent to all M SCs. Expand
Transient bullous dermolysis of the newborn associated with compound heterozygosity for recessive and dominant COL7A1 mutations.
TLDR
A TBDN patient is reported who is compound heterozygous for a recessive and a dominant glycine substitution mutation in COL7A1, which causes massive, transitory retention of collagen VII in the epidermis, its reduced deposition at the basement membrane zone, and extensive dermo-epidermal separation at birth. Expand
Establishing Criteria for Human Mesenchymal Stem Cell Potency
TLDR
The additional phenotypic criteria presented in this study when combined with the existing ISCT minimum criteria and working proposal will permit an improved assessment of MSC potency and provide a basis for establishing the quality of M SCs prior to their therapeutic application. Expand
Two Forms of Collagen XVII in Keratinocytes
TLDR
Collagen XVII is not only an unusual type II transmembrane collagen, but the first collagen with a specifically processed, soluble triple-helical ectodomain of collagen XVII, generated through furin-mediated proteolytic processing. Expand
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