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Genome-Wide Analysis of Factors Affecting Transcription Elongation and DNA Repair: A New Role for PAF and Ccr4-Not in Transcription-Coupled Repair
Evidence is provided that PAF and Ccr4-Not are required for efficientTC-NER in yeast, unraveling a novel function for these transcription complexes and opening new perspectives for the understanding of TC-NER and its functional interconnection with transcription elongation.
Arginine Catabolism in the CyanobacteriumSynechocystis sp. Strain PCC 6803 Involves the Urea Cycle and Arginase Pathway
The metabolic pathways proposed to explain [(14)C]arginine catabolism provide a rationale for understanding how nitrogen is made available to the cell after mobilization of cyanophycin [multi-L-arginyl-poly(L-aspartic acid)], a reserve material unique to cyanobacteria.
Identification of genes encoding amino acid permeases by inactivation of selected ORFs from the Synechocystis genomic sequence.
Genes encoding elements of four amino acid permeases were identified by insertional inactivation of ORFs from the genomic sequence of the cyanobacterium Synechocystis sp. strain PCC 6803 whose
An improved system for estradiol-dependent regulation of gene expression in yeast
The feedback regulatory loop that is engineered allows a 250-fold induction of the regulated gene, without increasing the basal activity of the target promoter, and achieving a 12-fold higher regulation efficiency than the previous configuration.
Systems for applied gene control in Saccharomyces cerevisiae
Saccharomyces cerevisiae is frequently used in biotechnology, including fermentative processes in food production, heterologous protein production and high throughput developments for biomedicine.
A novel yeast expression system based on a hormone-induced transcriptional cascade
A good alternative to regulate transcription driven by GAL promoters is the incorporation of the hybrid protein developed by D. Picard, which combines features of three different transcriptional activators, the DNA binding domain of Gal4, the hormone response domain of the human estrogen receptor (ER), and the transcription activation domain of herpes-virus VP16.
Abstract B11: Use of novel multigenic vectors to model complex genome-based colorectal cancer profiles in Drosophila
A novel multigenic vector that carries three different multiple cloning sites designed for cloning three separate transgenes to model complex genome-based colorectal cancer profiles in Drosophila is generated and it is shown that transgenic flies carrying synthetic clusters of as many as 16 hairpins can be generated.