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Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides
ABSTRACT Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture mediumExpand
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The lid is a structural and functional determinant of lipase activity and selectivity
In several lipases access to the enzyme active site is regulated by the position of a mobile structure named the lid. The role of this region in modulating lipase function is reviewed in this paperExpand
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Analytic versions of the zero divisor conjecture
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The first Lp-cohomology of some finitely generated groups and p-harmonic functions
Let G be a finitely generated infinite group and let p>1. In this paper we make a connection between the first Lp-cohomology space of G and p-harmonic functions on G. We also describe the elements inExpand
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Flavin Mononucleotide-Based Fluorescent Proteins Function in Mammalian Cells without Oxygen Requirement
Usage of the enhanced green fluorescent protein (eGFP) in living mammalian cells is limited to aerobic conditions due to requirement of oxygen during chromophore formation. Since many diseases orExpand
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ZERO DIVISORS AND LP(G)
Let G be a discrete group, CG the group ring of G over C and LP(G) the Lebesgue space of G with respect to Haar measure. It is known that if G is torsion free elementary amenable, 0 + a E CG and 0 =Expand
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Group cohomology and $L^p$-cohomology of finitely generated groups
Let $G$ be a finitely generated, infinite group, let $p>1$, and let $L^p(G)$ denote the Banach space $\{\sum_{x\in G} a_xx \mid \sum_{x\in G} |a_x |^p < \infty \}$. In this paper we will study theExpand
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Graphs of bounded degree and the p-harmonic boundary
Let p be a real number greater than one and let G be a connected graph of bounded degree. We introduce the p-harmonic boundary of G and use it to characterize the graphs G for which the constantExpand
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Learning from Directed Evolution: Further Lessons from Theoretical Investigations into Cooperative Mutations in Lipase Enantioselectivity
  • M. Reetz, M. Puls, +6 authors N. Otte
  • Medicine, Chemistry
  • Chembiochem : a European journal of chemical…
  • 2 January 2007
An earlier experimental study, which involved the directed evolution of enantioselective lipase variants from Pseudomonas aeruginosa as catalysts in the hydrolytic kinetic resolution ofExpand
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