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Cloning, expression, and nucleotide sequence of genes involved in production of pediocin PA-1, and bacteriocin from Pediococcus acidilactici PAC1.0
It was shown by subcloning of pSRQ11 in Escherichia coli cloning vectors that pediocin PA-1 is produced and, most probably, secreted by E. coli cells. Expand
Heterogeneity of the vanA gene cluster in clinical isolates of enterococci from the northeastern United States
It is suggested that the van cluster in recent isolates from the northeastern United States differs from that present in the early European VanA phenotype strains, and comprises a novel insertion-like sequence, IS1251. Expand
Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
The recent availability of bacterial genome sequence information permits the identification of conserved genes that are potential targets for novel antibiotic drug discovery and the same high-throughput methodology was used to overexpress gene products and to examine possible polarity effects for all essential genes. Expand
Dissemination among staphylococci of DNA sequences associated with methicillin resistance
DNA probes constructed from two methicillin-resistant S. aureus strains with different DNA sequences 5' to mecA suggest that the additional DNA responsible for the mecR1 deletion was part of a repetitive, and possibly mobile, element resident in coagulase-negative staphylococci but not in S.Aureus. Expand
Direct quantitation of the number of individual penicillin-binding proteins per cell in Escherichia coli
The penicillin-binding proteins (PBPs) are a set of enzymes that participate in the terminal stages of bacterial peptidoglycan assembly that also covalently bind and are inhibited by beta-lactam antibiotics. Expand
Comparison of cefepime, cefpirome, and cefaclidine binding affinities for penicillin-binding proteins in Escherichia coli K-12 and Pseudomonas aeruginosa SC8329
Despite differences in PBP binding, cefepime, ceppirome, and cefaclidine all displayed similar bactericidal activity for E. coli K-12 over the initial 3 h after antibiotic addition. Expand
Inhibition of Listeria monocytogenes by using bacteriocin PA-1 produced by Pediococcus acidilactici PAC 1.0
The bacteriocin produced by Pediococcus acidilactici PAC 1.0, previously designated PA-1 bacteriocin, was found to be inhibitory and bactericidal for Listeria monocytogenes. A dried powder preparedExpand
Characterization of IS1272, an insertion sequence-like element from Staphylococcus haemolyticus
IS1272, which appears to be primarily resident in S. haemolyticus, has disseminated to multiple staphylococcal species and is prevalent in multiresistant isolates and database searches yielded amino acid homology with proteins predicted to be encoded by open reading frames from a putative insertion sequence element from Enterococcus hirae. Expand
Role of mecA transcriptional regulation in the phenotypic expression of methicillin resistance in Staphylococcus aureus
Cloned mecR1-mecI repressed phenotypic expression of methicillin resistance, mecA transcription and PBP2a production and mediated P BP2a induction in response to certain beta-lactam antibiotics. Expand
In Vitro and In Vivo Activities of a Novel Cephalosporin, BMS-247243, against Methicillin-Resistant and -Susceptible Staphylococci
In conclusion, BMS-247243 has in vitro and in vivo activities against methicillin-resistant staphylococci and thus may prove to be useful in the treatment of infections caused by these multidrug-resistant organisms. Expand