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Immobilization and stabilization of recombinant multimeric uridine and purine nucleoside phosphorylases from Bacillus subtilis.
The derivatives prepared were successfully used in the synthesis of 2'-deoxyguanosine by enzymatic transglycosylation in aqueous solution between 2'- deoxyuridine and guanine. Expand
Immobilized penicillin G acylase as reactor and chiral selector in liquid chromatography.
The HPLC columns based on immobilized PGA combine the hydrolytic activity and the chiral recognition properties of PGA, therefore they have been used for the development of a combined reaction-separation system for chiral and achiral substrates. Expand
Molecular modeling and synthesis of inhibitors of herpes simplex virus type 1 uracil-DNA glycosylase.
Several hydrophilic derivatives were predicted and found to be active as UDG inhibitors, and these compounds will be useful to determine if UDG, like the viral thymidine kinase, is required for reactivation of HSV1 from latency in nerve cells. Expand
Major Depression and Bipolar Disorder: The Concept of Symmetry Breaking
In this paper we consider the results obtained by biochemical experimental data of platelet membrane fatty acids processed by a Self Organizing Map (SOM) from apparently healthy, bi-polar and majorExpand
High-performance liquid chromatography method for analyzing the antiretroviral agent efavirenz in human plasma.
The described assay is a rapid and accurate method for measurement of EFV in plasma: the easy preparation and small sample size makes this assay highly suitable for pharmacokinetic studies and routine clinical analysis in patients with HIV. Expand
Novel nonsubstrate inhibitors of human thymidine phosphorylase, a potential target for tumor-dependent angiogenesis.
Novel 6-(phenylalkylamino)uracil derivatives which, at micromolar concentrations, inhibit both catabolic and anabolic reactions of human TP in vitro are presented. Expand
Influence of the enzyme derivative preparation and substrate structure on the enantioselectivity of penicillin G acylase
Abstract The immobilization of an enzyme on solid support can give a catalyst with improved stability and catalytic properties compared to the native enzyme; in this work the enantioselectivity ofExpand
Synthesis of 2′‐Deoxynucleosides by Transglycosylation with New Immobilized and Stabilized Uridine Phosphorylase and Purine Nucleoside Phosphorylase
Multimeric uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) of Bacillus subtilis have been expressed from genes cloned in Escherichia coli, purified, characterized, immobilizedExpand
Metabolites of Alachlor in Water: Identification by Mass Spectrometry and Chemical Synthesis
This biodegradation study of alachlor in water was designed to assess the presence and the identity of metabolites. Alachlor was incubated in river water for 28 days. The pesticide and itsExpand
Depression, osteoporosis, serotonin and cell membrane viscosity between biology and philosophical anthropology
This work studies the depression-osteoporosis relationship, with the aim of focusing on depressive disorders that concern the quantitative dynamic of platelet membrane viscosity and interactome cytoskeleton modifications as a possible condition of the involvement of the serotonin axis (gut, brain and platelet), not only in depression but also in connection with osteoporotic. Expand