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Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances.
TLDR
A brief summary of the current knowledge on oleaginous algae and their fatty acid and TAG biosynthesis, algal model systems and genomic approaches to a better understanding of TAG production, and a historical perspective and path forward for microalgae-based biofuel research and commercialization are provided. Expand
The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing
TLDR
This Community Page describes a resource of 700 transcriptomes from marine microbial eukaryotes to help understand their role in the world's oceans. Expand
Genetic Engineering of Algae for Enhanced Biofuel Production
TLDR
Potential avenues of genetic engineering that may be undertaken in order to improve microalgae as a biofuel platform for the production of biohydrogen, starch-derived alcohols, diesel fuel surrogates, and/or alkanes are focused on. Expand
Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
TLDR
It is shown that N. gaditana has highly favourable lipid yields, and is a promising production organism, and the availability of a genome sequence and transformation methods will facilitate investigations into N. Gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga. Expand
Maturation of hydrogenases.
TLDR
A series of other gene products with intriguing putative functions indicate that the minimal pathway established for E. coli [NiFe]-hydrogenase maturation may possess even higher complexity in other organisms. Expand
Discovery of Two Novel Radical S-Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase*
TLDR
It is demonstrated here that HydEF and HydG function in the assembly of [Fe] hydrogenase, the first report on the nature of the accessory genes required for the maturation of an active [Fe]: radical S-adenosylmethionine superfamily of proteins. Expand
Immobilized gallium(III) affinity chromatography of phosphopeptides.
TLDR
A novel procedure for micropurification of phosphorylated peptides, as a front end to mass spectrometric analysis, is described, and the use of an immobilized metal affinity chromatography (IMAC) in a microtip in combination with Ga(III) ions is proposed. Expand
Anaerobic Acclimation in Chlamydomonas reinhardtii
TLDR
Overall, this study uses metabolite, genomic, and transcriptome data to provide genome-wide insights into the regulation of the complex metabolic networks utilized by Chlamydomonas under the anaerobic conditions associated with H2 production. Expand
Increased Lipid Accumulation in the Chlamydomonas reinhardtiista7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains
TLDR
The results demonstrate that both starchless mutants metabolize less acetate and have more severely attenuated levels of photosynthetic O2 evolution than CC124, indicating that a decrease in overall anabolic processes is a significant physiological response in the starch less mutants during nitrogen deprivation. Expand
Aquatic phototrophs: efficient alternatives to land-based crops for biofuels.
TLDR
This work proposes the use of biofuels derived from aquatic microbial oxygenic photoautotrophs, more commonly known as cyanobacteria, algae, and diatoms, to mitigate some of the potentially deleterious environmental and agricultural consequences associated with current land-based-biofuel feedstocks. Expand
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