The Molecular Architecture of Axonemes Revealed by Cryoelectron Tomography
- D. Nicastro, C. Schwartz, J. Pierson, R. Gaudette, M. Porter, J. McIntosh
- BiologyScience
- 18 August 2006
The three-dimensional structure of rapidly frozen axonemes from Chlamydomonas and sea urchin sperm is described using cryoelectron tomography and image processing to focus on the motor enzyme dynein, and images suggest a model for the way Dynein generates force to slide microtubules.
The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella
- T. Heuser, Milen Raytchev, Jeremy Krell, M. Porter, D. Nicastro
- BiologyJournal of Cell Biology
- 14 December 2009
Elegant cryoelectron tomography reveals that the nexin link between microtubule doublets in 9 + 2 axonemal structures, critical for their ability to bend, is the dynein regulatory complex.
Cytoplasmic dynein heavy chain 1b is required for flagellar assembly in Chlamydomonas.
- M. Porter, R. Bower, J. Knott, P. Byrd, W. Dentler
- BiologyMolecular Biology of the Cell
- 1 March 1999
The results indicate that cDhc1b is involved in the transport of components required for flagellar assembly in Chlamydomonas.
The 9 + 2 Axoneme Anchors Multiple Inner Arm Dyneins and a Network of Kinases and Phosphatases That Control Motility
Cilia and flagella are found on a variety of cell types, ranging from single cell protozoa and sperm to the ciliated epithelia of the respiratory and reproductive tracts. Despite this diversity, most…
The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport.
- Joshua Mueller, C. Perrone, R. Bower, D. Cole, M. Porter
- BiologyMolecular Biology of the Cell
- 1 March 2005
A ts mutation in the Chlamydomonas gene encoding KAP is characterized, which is required for the localization of Kinesin-2 at the site of flagellar assembly and the efficient transport of anterograde IFT particles within flagella.
Components of a "dynein regulatory complex" are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella
- L. Gardner, E. O'Toole, C. Perrone, T. Giddings, M. Porter
- BiologyJournal of Cell Biology
- 1 December 1994
The results suggest that some of the DRC components are located at the base of the second radial spoke in close association with the inner dynein arms, and this position is appropriate for a complex that is thought to mediate signals between the radial spokes and the dyne in arms.
The nexin-dynein regulatory complex subunit DRC1 is essential for motile cilia function in algae and humans
- Maureen Wirschell, H. Olbrich, H. Omran
- Biology, MedicineNature Genetics
- 27 January 2013
This work identifies the DRC1 subunit of the nexin-dynein regulatory complex (N-DRC), an axonemal structure critical for the regulation of dynein motors, and shows that mutations in the gene encoding DRC 1, CCDC164, are involved in PCD pathogenesis.
A Differential Cargo-Loading Model of Ciliary Length Regulation by IFT
- Kathryne Wren, Julie M. Craft, K. Lechtreck
- BiologyCurrent Biology
- 16 December 2013
The Shigella virulence gene regulatory cascade: a paradigm of bacterial gene control mechanisms
- Charles J. Dorman, M. Porter
- BiologyMolecular Microbiology
- 1 August 1998
A wealth of detailed information is now available on the Shigella virulence gene control circuits, and it is becoming clear that these share many features with regulatory systems found in other bacterial pathogens.
A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest–specific gene product
PF2 and its homologues appear to be universal components of motile axonemes that are required for DRC assembly and the regulation of flagellar motility and the expression of Gas8/Gas11 transcripts in a wide range of tissues may also indicate a potential role for PF2-related proteins in other microtubule-based structures.
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