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Use of cyclodextrins for manipulating cellular cholesterol content.
The methylated beta-cyclodextrin was found to be a more efficient donor in all cell types studied, with an average cholesterol uptake of at least 100 microg cholesterol/mg protein within 6 h. Expand
Molecular Mechanisms of Cellular Cholesterol Efflux*
  • M. Phillips
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 29 July 2014
Current understanding of the molecular mechanisms involved in these four efflux pathways for exporting free cholesterol to apolipoprotein A-I promoted by ABCA1 is presented in this minireview. Expand
From noncoding variant to phenotype via SORT1 at the 1p13 cholesterol locus
Functional evidence for a novel regulatory pathway for lipoprotein metabolism is provided and it is suggested that modulation of this pathway may alter risk for MI in humans. Expand
Cholesterol efflux and atheroprotection: advancing the concept of reverse cholesterol transport.
High-density lipoprotein (HDL) has been proposed to have several antiatherosclerotic properties, including the ability to mediate macrophage cholesterol efflux, antioxidant capacity, antiinflammatoryExpand
Cellular Cholesterol Efflux Mediated by Cyclodextrins (*)
The very high efficiency of cyclodextrins in stimulating cell cholesterol efflux suggests that these compounds can be used in two general ways for studies of atherosclerosis: 1) as research tools to probe mechanisms of cholesterol transport and aspects of membrane structure or 2) as potential pharmacological agents that could modify in vivo cholesterol metabolism and influence the development of the atherosclerotic plaque. Expand
Importance of different pathways of cellular cholesterol efflux.
The relative importance of the SR-BI and ABCA1 efflux pathways in preventing the development of atherosclerotic plaque is not known but will depend on the expression levels of the two proteins and on the type of cholesterol acceptors available. Expand
High-density lipoprotein heterogeneity and function in reverse cholesterol transport
This review summarizes the current understanding of how the biological activities of individual subclasses of HDL particles contribute to overall HDL performance in RCT and indicates that apolipoprotein A-I-containing nascent HDL particles are heterogeneous and that such particles exert different effects on the RCT pathway. Expand
Mechanism of ATP-binding Cassette Transporter A1-mediated Cellular Lipid Efflux to Apolipoprotein A-I and Formation of High Density Lipoprotein Particles*
A key feature of this mechanism is that membrane bending induced by ABCA1 lipid translocase activity creates the conditions required for nascent HDL assembly by apo A-I. Expand
Cellular Cholesterol Efflux Mediated by Cyclodextrins
It seems that cyclodextrin molecules are substantially more efficient than phospholipid acceptors, because cholesterol molecules desorbing from a membrane surface can diffuse directly into the hydrophobic core of a cyclodeXTrin molecule without having to desorb completely into the aqueous phase before being sequestered by the acceptor. Expand
High density lipoprotein structure-function and role in reverse cholesterol transport.
The abilities of apoA-I and apoE to solubilize phospholipid and stabilize HDL particles enable these proteins to be partners with ABCA1 in mediating efflux of cellular phospholIPid and cholesterol, and the biogenesis of HDL particles. Expand