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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
Comparative analysis of estrogenic activity in sewage treatment plant effluents involving three in vitro assays and chemical analysis of steroids
- Barbara V. Rutishauser, M. Pesonen, R. Eggen
- BiologyEnvironmental toxicology and chemistry
- 1 April 2004
All three in vitro systems, with the yeast‐based system being the easiest and most robust, are applicable for the screening of estrogenic activity in effluent samples.
Differential induction of cytochrome P-450-dependent monooxygenase, epoxide hydrolase, glutathione transferase and UDP glucuronosyl transferase activities in the liver of the rainbow trout by…
Effects of Xenoestrogenic Environmental Pollutants on the Proliferation of a Human Breast Cancer Cell Line (MCF-7)
- A. Blom, E. Ekman, A. Johannisson, L. Norrgren, M. Pesonen
- Chemistry, BiologyArchives of environmental contamination and…
- 1 April 1998
A human breast cancer cell line (MCF-7) was used to develop an in vitro screening assay for the detection of xenoestrogenic environmental pollutants and it may prove a valuable tool for screening potential estrogen-mimicking environmental pollutants.
Alkaline comet assay in rainbow trout hepatocytes.
Assessment of PCBs and Hydroxylated PCBs as Potential Xenoestrogens: In Vitro Studies Based on MCF-7 Cell Proliferation and Induction of Vitellogenin in Primary Culture of Rainbow Trout Hepatocytes
- P. Andersson, A. Blom, L. Norrgren
- Biology, ChemistryArchives of environmental contamination and…
- 1 August 1999
In both studies the hydroxylated PCBs were more active than the PCBs, and 4′-30 was the most active compound second only to 17β-estradiol.
Unbleached pulp mill effluents affect cytochrome P450 monooxygenase enzyme activities
Expression of P4501A1 in a primary culture of rainbow trout hepatocytes exposed to beta-naphthoflavone or 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Subcellular localization and properties of cytochrome P-450 and UDP glucuronosyltransferase in the rainbow trout kidney.
Environmental contaminants and biochemical responses in flatfish from the Hvaler Archipelago in Norway
- A. Goksøyr, A. Husøy, L. Förlin
- Environmental Science, BiologyArchives of environmental contamination and…
- 1 November 1991
The integrated chemical-biochemical approach employed in this study can obviously be expanded to give fruitful information about cause-effect relationships in other contaminant situations.