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Hydrophobins: the protein-amphiphiles of filamentous fungi.
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei.
Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei
- M. Ilmén, A. Saloheimo, M. Onnela, M. Penttilä
- BiologyApplied and environmental microbiology
- 1 April 1997
RNA slot blot experiments showed that no expression could be observed on glucose-containing medium and that high glucose levels abolish the inducing effect of sophorose, showing that distinct and clear-cut mechanisms of induction and glucose repression regulate cellulase expression in an actively growing fungus.
Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface
The real-time visualization of crystalline cellulose degradation by individual cellulase enzymes through use of an advanced version of high-speed atomic force microscopy is reported here.
Transcriptional regulation of plant cell wall degradation by filamentous fungi.
Metabolic engineering applications to renewable resource utilization.
Swollenin, a Trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materials.
A novel gene with sequence similarity to plant expansins, isolated from the cellulolytic fungus Trichoderma reesei, is described, which has an N-terminal fungal type cellulose binding domain connected by a linker region to the expansin-like domain.
Xylose transport studies with xylose-utilizing Saccharomyces cerevisiae strains expressing heterologous and homologous permeases
- A. Saloheimo, Jenita Rauta, O. Stasyk, A. Sibirny, M. Penttilä, L. Ruohonen
- Biology, EngineeringApplied Microbiology and Biotechnology
- 1 April 2007
Xylose uptake properties of a recombinant xylose-utilizing yeast Saccharomyces cerevisiae are modified by expression of heterologous and homologous permease-encoding genes and the new transporter homologue, Trxlt1 thus appears to code for a protein specific for xylOSE uptake.
ACEI of Trichoderma reesei Is a Repressor of Cellulase and Xylanase Expression
- N. Aro, M. Ilmén, A. Saloheimo, M. Penttilä
- Biology, EngineeringApplied and Environmental Microbiology
- 1 January 2003
The effect of deletion of the Trichoderma reesei ace1 gene encoding the novel cellulase regulator ACEI resulted in an increase in the expression of all the main cellulase genes and two xylanase genes in sophorose- and cellulose-induced cultures, indicating that ACEI acts as a repressor of cellulase and xylanases expression.
ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei *
- N. Aro, A. Saloheimo, M. Ilmén, M. Penttilä
- Biology, EngineeringThe Journal of Biological Chemistry
- 29 June 2001
A novel yeast-based method to isolate transcriptional activators was applied to clone regulators binding to the cellulase promoter cbh1 of the filamentous fungusTrichoderma reesei (Hypocrea…