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Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate.
TLDR
Rhodococcus rhodochrous haloalkane dehalogenase mutants with up to 32-fold higher activity than wild type are obtained toward the toxic, recalcitrant anthropogenic compound 1,2,3-trichloropropane using a new strategy that identifies key residues in access tunnels connecting the buried active site with bulk solvent by rational design and randomized them by directed evolution. Expand
Modification of Activity and Specificity of Haloalkane Dehalogenase from Sphingomonas paucimobilis UT26 by Engineering of Its Entrance Tunnel*
TLDR
The strategy of using site-directed mutagenesis to modify a specific entrance tunnel residue identified by structural and phylogenetic analyses, rather than combinatorial screening, generated a high percentage of viable mutants. Expand
Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations.
TLDR
It is proposed that engineering the accessibility of tunnels and the mechanisms of ligand exchange is a powerful strategy for modification of the functional properties of enzymes with buried active sites. Expand
Cloning, Biochemical Properties, and Distribution of Mycobacterial Haloalkane Dehalogenases
TLDR
The cloning of the haloalkane dehalogenase genes dmbA and dmbB from M. bovis are described and the dehalagenase activity of their translation products are demonstrated and the evolution and distribution of h HaloalkaneDehalogenases among mycobacteria is discussed. Expand
A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation
TLDR
The results demonstrate the successful use of a laboratory-evolved dehalogenase and genetic engineering to produce an effective, plasmid-free, and stable whole-cell biocatalyst for the aerobic bioremediation of a recalcitrant chlorinated hydrocarbon. Expand
Quantitative structure-activity relationships for toxicity and genotoxicity of halogenated aliphatic compounds: wing spot test of Drosophila melanogaster.
TLDR
Nucleophilic superdelocalizability calculated by quantum mechanics appears to be a good parameter for prediction of both toxicity and genotoxicity effects of halogenated aliphatic compounds. Expand
Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis.
TLDR
The enzyme DhaA from Rhodococcus rhodochrous NCIMB 13064 belongs to the h Haloalkane dehalogenases, which catalyze the hydrolysis of haloalkanes to the corresponding alcohols, and can be used to detoxify the industrial pollutant 1,2,3-trichloropropane (TCP). Expand
The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: reaction mechanism and molecular evolution.
Haloalkane dehalogenase DhmA from Mycobacterium avium N85 showed poor expression and low stability when produced in Escherichia coli. Here, we present expression DhmA in newly constructed pK4RPExpand
Mycobacterial Haloalkane Dehalogenases: Cloning, BiochemicalProperties and distribution.
TLDR
The cloning of the haloalkane dehalogenase genes dmbA and dmbB from M. bovis 5033/66 are described and the dehalagenase activity of their translation products are proved and Evolution and distribution of h HaloalkaneDehalogenases among mycobacteria is discussed. Expand
Structure-Based Engineering of Haloalkane Dehalogenase
Poster presented by Mgr.Marta Monincova on Conference Genetics of Industrial Microorganisms! in Prague.
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