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Peripheral and Integral Binding of Cytochrome c to Phospholipids Vesicles
The interactions of ferric cytochrome c (Cyt-c) with dioleoyl-phosphatidylglycerol (DOPG) at low ionic strength have been studied by viscosity and turbidity measurements as well as by resonanceExpand
Vesicle reconstitution from lipid-detergent mixed micelles.
The process of formation of lipid vesicles using the technique of detergent removal from mixed-micelles is examined and new methods inducing micelle-vesicle transition using enzymatic reaction and T-jump are described and compared to more classical ones. Expand
Laurdan solvatochromism: solvent dielectric relaxation and intramolecular excited-state reaction.
The observation of an isoemissive point in the temperature range from -50 degrees C to -110 degrees C in ethanol suggests an interconvertion between two average excited-state populations: unrelaxed and solvent-relaxed CT states and a possible scheme is proposed for the deexcitation pathway, taking into account the kinetics observed in these different solvents. Expand
Solubilisation of dipalmitoylphosphatidylcholine bilayers by sodium taurocholate: a model to study the stability of liposomes in the gastrointestinal tract and their mechanism of interaction with a
The addition of DPPC appears an attractive strategy to formulate orally administered liposomes because of the lower insertion of TC into DPPC bilayer at 37 degrees C at low TC concentration in the medium (fasted conditions). Expand
A comparative study of the action of melittin on sphingomyelin and phosphatidylcholine bilayers
It was found that melittin-induced macroscopic changes of sphingomyelin membranes are qualitatively the same as in the case of dipalmitoylphosphatidylcholine bilayers, and the sphingomers/melittin system is proposed to show a reversible vesicle-to-disc transition through an intermediate fusion or aggregation event centered at the main transition temperature, Tm. Expand
New insights into pb5, the receptor binding protein of bacteriophage T5, and its interaction with its Escherichia coli receptor FhuA.
It is shown that unlike RBPs of known structures, pb5 probably folds as a unique domain fulfilling both functions of binding to the host receptor and interaction with the rest of the phage, which is the first structural information regarding the binding of a RBP to a proteic receptor. Expand
Liposomes: Preparation and Membrane Protein Reconstitution
Liposomes are hollow microspheres, their membrane is composed of one or several lipid bilayers and encapsulates a small volume of the medium in which they have been prepared. This morphology enablesExpand
Partition coefficient of a surfactant between aggregates and solution: application to the micelle-vesicle transition of egg phosphatidylcholine and octyl beta-D-glucopyranoside.
The mechanism of the solubilization of egg phosphatidylcholine containing 10% (M/M) of egg phosphatidic acid unilamellar vesicles by the nonionic detergent, octyl beta-D-glucopyranoside, has beenExpand
Size analysis and stability study of lipid vesicles by high-performance gel exclusion chromatography, turbidity, and dynamic light scattering.
High-performance liquid chromatography analysis of REV's demonstrates that both the average size and polydispersity of the vesicles decrease with decreasing pore size of the membranes, cellulosic or PVF "tortuous" ones being less efficient than "straight bores" polycarbonate ones. Expand
Elimination and exchange of trifluoroacetate counter‐ion from cationic peptides: a critical evaluation of different approaches
Three different approaches to exchange the tightly bound TF‐acetate counter‐ion from the dicationic octapeptide lanreotide are tested: reverse‐phase HPLC, ion‐exchange resin, and deprotonation/reprotonation cycle of the amino groups. Expand