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Swollenin, a Trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materials.
TLDR
A novel gene with sequence similarity to plant expansins, isolated from the cellulolytic fungus Trichoderma reesei, is described, which has an N-terminal fungal type cellulose binding domain connected by a linker region to the expansin-like domain.
Monoclonal antibodies against core and cellulose-binding domains of Trichoderma reesei cellobiohydrolases I and II and endoglucanase I.
TLDR
Monoclonal antibodies can be specifically used to recognize and quantitate different domains of these three important cellulolytic enzymes from Trichoderma reesei.
The cloning and sequencing of the genes encoding phytase (phy) and pH 2.5-optimum acid phosphatase (aph) from Aspergillus niger var. awamori.
The genes encoding phytase (EC 3.1.3.8) and pH 2.5-optimum acid phosphatase (EC 3.1.3.2) have been cloned and sequenced from Aspergillus niger var. awamori. The translated nucleotide sequences
Thermostable xylanases, Xyn10A and Xyn11A, from the actinomycete Nonomuraea flexuosa: isolation of the genes and characterization of recombinant Xyn11A polypeptides produced in Trichoderma reesei
TLDR
Two endoxylanases, cloned from a Nonomuraea flexuosa (previously Actinomadura flexUosa) DSM43186 genomic expression library in Escherichia coli, and recombinant Xyn11A expressed in the filamentous fungus Trichoderma reesei, were purified from cultivation media and characterized.
Genetic Modification of Carbon Catabolite Repression in Trichoderma reesei for Improved Protein Production
TLDR
The results of this work indicate that cre1 is a valid target gene in strain engineering for improved enzyme production in T. reesei and that the cre1-1 gene is practically a null allele.
Enhanced production of cellobiohydrolases in Trichoderma reesei and evaluation of the new preparations in biofinishing of cotton.
TLDR
When the enzyme preparation with elevated CBHII content was used in biofinishing of cotton, better depilling and visual appearance were achieved than with the wild type preparation; however, the improvement was not as pronounced as with preparations with elevated levels of endoglucanases (EG).
Production of cyclomaltodextrin glucanotransferase of Bacillus circulans var. alkalophilus ATCC21783 in B. subtilis
TLDR
To achieve efficient enzyme production in B. subtilis, the gene was placed under the control of the B. amyloliquefaciens α-amylase promoter and both of the plasmid constructions were stable when grown over 50 generations without antibiotic selection.
A New Subtilase-Like Protease Deriving from Fusarium equiseti with High Potential for Industrial Applications
TLDR
It is considered that the F. equiseti protease (Fe protease) showed excellent performance in stain removal and good compatibility with several commercial laundry detergent formulations, suggesting that it has high potential for use in various industrial applications.
Nucleotide Sequence of the α-Amylase-Pullulanase Gene from Clostridium Thermohydrosulfuricum
TLDR
The deduced sequence of the mature apu product showed similarities to various amylolytic enzymes, whereas the signal sequence showed similarity to those of the α-amylases of B. stearothermophilus and B. subtilis.
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