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Genetics of a novel starch utilisation pathway present in Klebsiella oxytoca.
A 14.3 kb DNA fragment from Klebsiella oxytoca M5a1 has been cloned and shown to provide Escherichia coli with the capacity for growth on alpha- and beta-cyclodextrins. This fragment is locatedExpand
Metabolism of cyclodextrins byKlebsiella oxytoca M5a1: purification and characterisation of a cytoplasmically located cyclodextrinase
It has been shown previously that the products of 11 genes are required for metabolism of starch byKlebsiella oxytoca via a novel pathway. An extracellular cyclodextrin glucanotransferase firstExpand
The periplasmic cyclodextrin binding protein CymE from Klebsiella oxytoca and its role in maltodextrin and cyclodextrin transport.
Klebsiella oxytoca M5a1 has the capacity to transport and to metabolize alpha-, beta- and gamma-cyclodextrins. Cyclodextrin transport is mediated by the products of the cymE, cymF, cymG, cymD, andExpand
Properties of a Cyclodextrin-specific, Unusual Porin from Klebsiella oxytoca *
The function of CymA, 1 of the 10 gene products involved in cyclodextrin uptake and metabolism by Klebsiella oxytoca, was characterized. CymA is essential for growth on cyclodextrins, but it can alsoExpand
CymA of Klebsiella oxytoca outer membrane: binding of cyclodextrins and study of the current noise of the open channel.
CymA, the outer membrane component of the cyclodextrin (CD) uptake and metabolism system of Klebsiella oxytoca, was reconstituted into lipid bilayer membranes. The channel properties of this unusualExpand
Enzymatic preparation of radiolabeled linear maltodextrins and cyclodextrins of high specific activity from [14C] maltose using amylomaltase, cyclodextrin glucosyltransferase and cyclodextrinase.
Radiolabeled linear and cyclic maltodextrins of high specific radioactivity were prepared using enzymes involved in maltodextrin metabolism. 14C-Labeled maltose was the starting material yieldingExpand
Mathematical treatment of transport data of bacterial transport systems to estimate limitation in diffusion through the outer membrane.
Bacterial transport systems are traditionally treated as enzymes exhibiting a saturable binding site giving rise to an apparent K(m)of transport, whereas the maximal rate of transport is regardedExpand
A process for producing an active heterodimeric AMW-RT in prokaryotic cells
In the present invention, the heterologous expression of the reserve transcriptase from the avian myeloblastosis virus (AMV-RT) in prokaryotic cells, in particular Escherichia coli (E. coli)Expand
Recombinant terminal deoxynucleotidyl with improved functionality
Truncated terminal deoxynucleotidyl transferase (TdT) derivative from calf thymus, characterized in that the derivative in comparison to the native TdT up to 161 amino acids N-terminally shortenedExpand