DENTAL ENAMEL FORMATION AND IMPLICATIONS FOR ORAL HEALTH AND DISEASE.
Key aspects of dental enamel formation are examined, from its developmental genesis to the ever-increasing wealth of data on the mechanisms mediating ionic transport, as well as the clinical outcomes resulting from abnormal ameloblast function.
Structural characterization of the major extrapallial fluid protein of the mollusc Mytilus edulis: implications for function.
Sequence comparisons reveal that the EP protein possesses little homology to any presently known matrix proteins previously isolated from mollusc shells but rather it highly resembles a heavy metal binding protein and a histidine-rich glycoprotein, both from the hemolymph of M. edulis.
Biological Organization of Hydroxyapatite Crystallites into a Fibrous Continuum Toughens and Controls Anisotropy in Human Enamel
- S. White, W. Luo, M. Paine, H. Fong, M. Sarikaya, M. Snead
- Materials ScienceJournal of dentistry research
- 1 January 2001
Measurements suggest that enamel is a composite ceramic with the crystallites oriented in a complex three-dimensional continuum, suggesting that inclusion of biological contaminants, such as protein, influences the properties of enamel.
Requirements for ion and solute transport, and pH regulation during enamel maturation
Estimation of relative mRNA levels of individual SLC, Cftr, and CAs in enamel cells obtained from secretory and maturation stages on rat incisors shows that the expression levels of multiple genes regulating extracellular pH are modulated during enamel maturation in response to an increased need for pH buffering during hydroxyapatite crystal growth.
New Paradigms on the Transport Functions of Maturation-stage Ameloblasts
- R. Lacruz, C. Smith, I. Kurtz, M. Hubbard, M. Paine
- BiologyJournal of dentistry research
- 1 February 2013
The main cellular activities of these genes during the maturation stage of amelogenesis are described, which include ion transport and storage, control of intracellular and extracellular pH, and endocytosis.
Enamel matrix proteins; old molecules for new applications.
- S. Lyngstadaas, J. Wohlfahrt, S. Brookes, M. Paine, M. Snead, J. Reseland
- BiologyOrthodontics & craniofacial research
- 1 August 2009
New experiments now confirm that amelogenins have potential for being used also in the fields of endodontics, bone regeneration, implantology, traumatology, and wound care.
Role of NBCe1 and AE2 in Secretory Ameloblasts
The data are the first evidence that AE2 and NBCe1 are expressed in ameloblasts in vivo in a polarized fashion, thereby providing a mechanism for ameloblast transcellular bicarbonate secretion in the process of enamel formation and maturation.
Comparison of two mouse ameloblast-like cell lines for enamel-specific gene expression
Comparing the expression profiles of enamel-specific genes in two mouse derived ameloblast-like cell lines provides greater insight as to the suitability of both cell lines to contribute to biological studies on enamel formation and biomineralization, and highlights some of the strengths and weaknesses.
Adaptor protein complex 2–mediated, clathrin‐dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis
Using real‐time PCR, it is shown that the expression of clathrin and adaptor protein subunits are upregulated in maturation stage rodent enamel organ cells, supporting the existence of a membrane‐bound receptor‐regulated pathway for the endocytosis of enamel matrix proteins.
The Circadian Clock Modulates Enamel Development
The hypothesis that the circadian clock temporally regulates enamel development is supported by data supporting the expression of the circadian transcription factors Per2 and Bmal1 and Nfya expression in serum-synchronized murine ameloblasts oscillated with a strong 24-hour rhythm.