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Structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase complex
TLDR
The structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF complex suggests that Cul1 may contribute to catalysis through the positioning of the substrate and the ubiquitin-conjugating enzyme, and this model is supported by Cul1 mutations designed to eliminate the rigidity of the scaffold. Expand
Deregulated proteolysis by the F-box proteins SKP2 and β-TrCP: tipping the scales of cancer
TLDR
The functionality and biology of the F-box proteins, SKP2 (S-phase kinase-associated protein 2) and β-TrCP (β-transducin repeat-containing protein), are explored, which are emerging as important players in cancer biogenesis owing to the deregulated proteolysis of their substrates. Expand
Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018
TLDR
An updated classification of cell death subroutines focusing on mechanistic and essential aspects of the process is proposed, and the utility of neologisms that refer to highly specialized instances of these processes are discussed. Expand
SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27
TLDR
It is shown that the F-box protein SKP2 specifically recognizes p27 in a phosphorylation-dependent manner that is characteristic of an F- box-protein–substrate interaction and is subject to dual control by the accumulation of bothSKP2 and cyclins following mitogenic stimulation. Expand
The SCF ubiquitin ligase: insights into a molecular machine
TLDR
A unifying and structurally detailed view of SCF-mediated proteolytic control of cellular processes that has been revealed by recent studies is explored. Expand
Human cyclin E, a nuclear protein essential for the G1-to-S phase transition
TLDR
It is found that mammalian cells express two forms of cyclin E protein which differ from each other by the presence or absence of a 15-amino-acid amino-terminal domain, and cyclins E and D1 control two different transitions within the human cell cycle. Expand
Insights into SCF ubiquitin ligases from the structure of the Skp1–Skp2 complex
TLDR
The structure raises the possibility that different Skp1 family members evolved to function with different subsets of F-box proteins, and suggests that the F- box protein may not only recruit substrate, but may also position it optimally for the ubiquitination reaction. Expand
Control of the SCF(Skp2-Cks1) ubiquitin ligase by the APC/C(Cdh1) ubiquitin ligase.
TLDR
The induction of Skp2 and Cks1 degradation in G1 represents a principal mechanism by which APC/C(Cdh1) prevents the unscheduled degradation of SCF(Skp2-Cks1) substrates and maintains the G1 state. Expand
Mechanisms and function of substrate recruitment by F-box proteins
TLDR
The evolution of substrate recruitment by F-box proteins, the dysregulation of substrates in disease and potential avenues for F- box protein-directed disease therapies are focused on. Expand
Cyclin A is required at two points in the human cell cycle.
TLDR
It is found that DNA synthesis and entry into mitosis are inhibited in human cells microinjected with anti‐cyclin A antibodies at distinct times, suggesting that cyclin A defines novel control points of the human cell cycle. Expand
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