Structural basis of the drug-binding specificity of human serum albumin.
Practical aspects of the ligand-binding and enzymatic properties of human serum albumin.
- U. Kragh-Hansen, V. Chuang, M. Otagiri
- Biology, ChemistryBiological and Pharmaceutical Bulletin
- 1 June 2002
A tabulation of high-affinity binding sites for both endogenous and exogenous compounds has been made; it could be useful for the above-mentioned purpose, but it could also be of value when trying to predict potential drug interactions at the protein-binding level.
Characterization of uremic toxin transport by organic anion transporters in the kidney.
- Tsuneo Deguchi, H. Kusuhara, A. Takadate, H. Endou, M. Otagiri, Y. Sugiyama
- Medicine, ChemistryKidney International
ROat1/hOAT1 and rOat3/ hOAT3 play major roles in the renal uptake of uremic toxins on the basolateral membrane of the proximal tubules.
p-Cresyl sulfate causes renal tubular cell damage by inducing oxidative stress by activation of NADPH oxidase.
The renal toxicity of PCS is attributed to its intracellular accumulation, leading to both increased NADPH oxidase activity and ROS production, which, in turn, triggers induction of inflammatory cytokines involved in renal fibrosis.
Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat model.
- Katsuhisa Inoue, Y. Nakai, H. Yuasa
- BiologyAmerican Journal of Physiology - Gastrointestinal…
- 1 March 2008
This study is the first to clone rPCFT/HCP1, and it successfully provided several lines of evidence that indicate its role as the molecular entity of the carrier-mediated intestinal folate transport system.
Molecular Cloning and Functional Analyses of OAT1 and OAT3 from Cynomolgus Monkey Kidney
Results suggest that monkey is a good predictor of the renal uptake of organic anions in the human and species differences in the OAT1- and OAT3-mediated transport between monkey and human are elucidated.
Role of blood–brain barrier organic anion transporter 3 (OAT3) in the efflux of indoxyl sulfate, a uremic toxin: its involvement in neurotransmitter metabolite clearance from the brain
Results suggest that OAT3 mediates the brain‐to‐blood transport of indoxyl sulfate, and is also involved in the efflux transport of neurotransmitter metabolites and drugs in the brain.
Functional Characterization of Human Proton-Coupled Folate Transporter/Heme Carrier Protein 1 Heterologously Expressed in Mammalian Cells as a Folate Transporter
It is found that sulfasalazine is a potent inhibitor of hPCFT/HCP1, which would interfere with the intestinal absorption of MTX when coadministered in therapy for rheumatoid arthritis as well as folate.
Role of arg-410 and tyr-411 in human serum albumin for ligand binding and esterase-like activity.
- H. Watanabe, S. Tanase, K. Nakajou, T. Maruyama, U. Kragh-Hansen, M. Otagiri
- Chemistry, BiologyBiochemical Journal
- 1 August 2000
In addition to its unique ligand binding properties, HSA also possesses an esterase-like activity, and studies with p-nitrophenyl acetate as a substrate showed that, although Arg-410 is important, the enzymic activity of HSA is much more dependent on the presence of Tyr-411.
Cytotoxicity of absorption enhancers in Caco-2 cell monolayers.
Results indicate that of the three classes of enhancer, dipotassium glycyrrhizinate was not cytotoxic and that high concentrations of sodium caprate and sodium deoxycholate could damage plasma and nuclear membranes.