M. Otagiri

Publications15
h-index7
Citations194
Highly Influential Citations15
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Structural and Biochemical Analyses of Glycoside Hydrolase Family 26 β-Mannanase from a Symbiotic Protist of the Termite Reticulitermes speratus*
Background: Symbiotic protists of the termite gut contribute to lignocellulosic biomass degradation. Results: A novel protistan β-mannanase efficiently degrades glucomannan and displays… Expand
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High-throughput recombinant gene expression systems in Pichia pastoris using newly developed plasmid vectors.
We describe here the construction of Gateway-compatible vectors, pBGP1-DEST and pPICZα-DEST, for rapid and convenient preparation of expression plasmids for production of secretory proteins in Pichia… Expand
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Structural basis for chiral substrate recognition by two 2,3‐butanediol dehydrogenases
2,3‐Butanediol dehydrogenase (BDH) catalyzes the NAD‐dependent redox reaction between acetoin and 2,3‐butanediol. There are three types of homologous BDH, each stereospecific for both substrate and… Expand
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Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unusual Na(+)/H(+) antiporter encoded by mrpABCDEFG. Using His tagging of Mrp proteins, we showed complex formation by the… Expand
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Cloning, expression and nucleotide sequence of the L-2,3-butanediol dehydrogenase gene from Brevibacterium saccharolyticum C-1012
Abstract A 3-kbp DNA fragment including the l -2,3-butanediol dehydrogenase ( l -BDH) gene ( bud C) from the chromosomal DNA of Brevibacterium saccharolyticum C-1012 was cloned in Escherichia coli… Expand
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The GH26 β-mannanase RsMan26H from a symbiotic protist of the termite Reticulitermes speratus is an endo-processive mannobiohydrolase: heterologous expression and characterization.
Symbiotic protists in the gut of termites are prominent natural resources for enzymes involved in lignocellulose degradation. Here we report expression, purification, and biochemical characterization… Expand
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Novel squalene-producing thraustochytrids found in mangrove water
On extended screening of squalene-producing strains in Okinawa mangrove water, we identified 14 novel squalene-producing thraustochytrids from 172 unialgal clonal isolates. The novel thraustochytrids… Expand
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Functional Expression of Three Rieske Non-Heme Iron Oxygenases Derived from Actinomycetes in Rhodococcus Species for Investigation of Their Degradation Capabilities of Dibenzofuran and Chlorinated…
The activity of Rieske non-heme iron oxygenases (aromatic hydrocarbon dioxygenases, AhDOs) is important for the bacterial degradation of aromatic pollutants such as polycyclic aromatic hydrocarbons… Expand
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The MrpA, MrpB and MrpD subunits of the Mrp antiporter complex in Bacillus subtilis contain membrane-embedded and essential acidic residues.
Bacillus subtilis Mrp is a unique Na+/H+ antiporter with a multicomponent structure consisting of the mrpABCDEFG gene products. We have previously reported that the conserved and putative… Expand
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Heterologous Expression and Characterization of a Glycoside Hydrolase Family 45 endo-β-1,4-Glucanase from a Symbiotic Protist of the Lower Termite, Reticulitermes speratus
The termite symbiotic system is one of the efficient lignocellulose degradation systems. We tried to express and characterize a novel cellulolytic enzyme from this system. Here, we report the… Expand
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