• Publications
  • Influence
Crystal Structure and Functional Analysis of the SARS-Coronavirus RNA Cap 2′-O-Methyltransferase nsp10/nsp16 Complex
TLDR
Two other crystal structures are presented, the inhibitor Sinefungin bound in the S-adenosylmethionine binding pocket and the tighter complex nsp10(Y96F)/nsp16, providing the first structural insight into the regulation of RNA capping enzymes in (+)RNA viruses. Expand
Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus.
TLDR
A comparison of both structures, the mutagenesis data and the analysis of GH68 family multiple sequences alignment show a strong conservation of the sucrose hydrolytic machinery among levansucrases and also a structural equivalence of the Bs levanucrase Ca2+-binding site to the LsdA Cys339-Cys395 disulphide bridge, suggesting similar fold-stabilizing roles. Expand
PknB kinase activity is regulated by phosphorylation in two Thr residues and dephosphorylation by PstP, the cognate phospho‐Ser/Thr phosphatase, in Mycobacterium tuberculosis
TLDR
Results indicate that, as for eukaryotic homologues, phosphorylation of the activation loop provides a regulation mechanism of mycobacterial kinases and strongly suggest that PknB and PstP could work as a functional pair in vivo to control myCobacterial cell growth. Expand
Crystal Structure of the Catalytic Domain of the PknB Serine/Threonine Kinase from Mycobacterium tuberculosis *
TLDR
The crystal structure of an active form of PknB, one of the four M. tuberculosis kinases that are conserved in the downsized genome of Mycobacterium leprae, is reported, revealing an enzyme in the active state with an unprecedented arrangement of the Gly-rich loop associated with a new conformation of the nucleotide γ-phosphoryl group. Expand
Structure of lactococcal phage p2 baseplate and its mechanism of activation
TLDR
Analysis of the p2 phage baseplate structure by X-ray crystallography and electron microscopy and propose a mechanism for the baseplate activation during attachment to the host cell reveal a novel siphophage activation and host-recognition mechanism leading ultimately to DNA ejection. Expand
Towards a Structural Comprehension of Bacterial Type VI Secretion Systems: Characterization of the TssJ-TssM Complex of an Escherichia coli Pathovar
TLDR
The crystal structure of the enteroaggregative Escherichia coli Sci1 TssJ lipoprotein, a two four-stranded β-sheets protein that exhibits a transthyretin fold with an additional α-helical domain and a protruding loop, is reported to provide an improved understanding of T6SS assembly and encourage structure-aided drug design of novel antimicrobials targeting T 6SS. Expand
Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism
TLDR
In vivo infection experiments confirmed that Ca2+ ions are required for host adhesion among p2-like phages but have no influence on TP901-1- like phages (P335-species), suggesting that these two families rely on diverse adhesion strategies which may lead to different signaling for genome release. Expand
Insights into the inter-ring plasticity of caseinolytic proteases from the X-ray structure of Mycobacterium tuberculosis ClpP1.
TLDR
It was found that disorder in the handle domain affects the formation and configuration of the tetradecamer and results in condensed structures with larger equatorial pores when compared with ClpPs from other species. Expand
Structural basis for DNA recognition and loading into a viral packaging motor
TLDR
Binding in vitro is probed by using EPR and surface plasmon resonance experiments, which indicated that interaction with DNA is mediated exclusively by the DNA-binding domains and suggested a nucleosome-like model in which DNA binds around the outside of the protein oligomer. Expand
Crystal Structure of the VgrG1 Actin Cross-linking Domain of the Vibrio cholerae Type VI Secretion System
TLDR
It is shown that less than 100 ACD molecules are sufficient to depolymerize the actin filaments of a fibroblast cell in vivo, and co-crystals with divalent cations and ATP reveal the molecular mechanism of the MARTX/VgrG toxins and offer perspectives for their possible inhibition. Expand
...
1
2
3
4
5
...