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Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study.
Combinations of appropriate concentrations of MgCl2 and CaCl2 were significantly effective in the reversion as well as in the stability of protoplasts. Expand
A plasmid involved in chloramphenicol production in Streptomyces venezuelae: evidence from genetic mapping.
It is concluded that chloramphenicol production is controlled by a plasmid that appeared to be non-transferable in conjugation. Expand
Molecular phylogeny of the order Euryalida (Echinodermata: Ophiuroidea), based on mitochondrial and nuclear ribosomal genes.
The existing taxonomy of Euryalida, one of the two orders of the Ophiuroidea (Echinodermata), is uncertain and characterized by controversial delimitation of taxonomic ranks from genus toExpand
Molecular phylogeny based on increased number of species and genes revealed more robust family-level systematics of the order Euryalida (Echinodermata: Ophiuroidea).
A family-level revision of the Euryalida based on sequences from mitochondrial genes (16S rRNA and COI) and a nuclear gene (18S r RNA) from 83 euryalid ophiuroids is presented. Expand
Non-destructive morphological observations of the fleshy brittle star, Asteronyx loveni using micro-computed tomography (Echinodermata, Ophiuroidea, Euryalida)
The first morphological observation of a euryalid brittle star, Asteronyx loveni, using non-destructive X-ray micro-computed tomography (µCT) was performed and shape and positional arrangement of taxonomically important ossicles were clearly observed without any damage to the body. Expand
Methods for the Determination of Deoxyribonucleic Acid Homologies in Streptomyces
Incubation of DNA-loaded filters in a solution of 50% formamide containing 6x standard saline citrate, at 48 C for 40 hr, was judged to be the best set of conditions tested based on relatively good retention of immobilized DNA, very low hybridization with unrelated DNA of a similarly high T(m) (from Sarcina lutea), and the formation of complexes similar in thermal stability to the native DNA. Expand
An evaluation of taxonomic criteria in streptomycetes on the basis of deoxyribonucleic acid homology.
The suitability of several diagnostic features as taxonomic criteria are discussed, and the DNAs of streptomycetes in the largest subgroup were found to be highly homologous to DNA from S. californicus ISP 5058 located in that subgroup. Expand
Construction of plasmid vectors from Streptomyces kasugaensis plasmids, pSK1 and pSK2.
Streptomyces kasugaensis G3 was transformed by pIJ702 DNA carrying the thiostrepton-resistance gene at a frequency of 2 X 10(5) transformants/micrograms DNA, but it was found that the introducedExpand
Function of plasmids in the production of aureothricin. I. Elimination of plasmids and alteration of phenotypes caused by protoplast regeneration in Streptomyces kasugaensis.
Plasmid curing in RII type strains and also the variation of plasmid copy in the RI type strains occurred as the result of protoplast regeneration, and the structural genes for biosynthesis of AT probably exist on chromosome. Expand