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PEGylated DNA/transferrin–PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery
We investigated the in vitro and in vivo properties of DNA/transferrin–polyethylenimine (800 kDa) complexes before and after covalent coupling of poly(ethylene glycol) (PEG). Upon incubation withExpand
Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer
Nonviral vectors based on polyethylenimine (PEI) usually contain an excess of PEI that is not complexed to DNA. Since unbound PEI contributes to cellular and systemic toxicity, purification ofExpand
The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells
Under physiological salt concentration, plasmid DNA complexed with transferrin-conjugated or unmodified polyethylenimine (PEI, 800 kDa) forms huge (up to >1000 nm) aggregates, unless the individualExpand
Novel shielded transferrin-polyethylene glycol-polyethylenimine/DNA complexes for systemic tumor-targeted gene transfer.
Tumor-targeting DNA complexes which can readily be generated by the mixing of stable components and freeze-thawed would be very advantageous for their subsequent application as medical products.Expand
Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery
Recently the high transfection potential of the cationic polymer polyethylenimine (PEI) was described (Boussif O et al. Proc Natl Acad Sci USA 1995; 92: 7297–7301). To combine the promising DNAExpand
Importance of lateral and steric stabilization of polyelectrolyte gene delivery vectors for extended systemic circulation.
Gene therapy for systemic diseases requires intravenous administration, but existing vectors are not suitable for systemic delivery, often showing rapid elimination from the bloodstream thatExpand
The internalization route resulting in successful gene expression depends on both cell line and polyethylenimine polyplex type.
Understanding cellular uptake and intracellular processing of nonviral gene delivery systems is a key aspect in developing more efficient vectors. In this study, the impact of clathrin- andExpand
Targeting tumors with non-viral gene delivery systems.
Targeting therapeutic genes to tumors is an attractive concept in curing malignant diseases. Systemic gene delivery systems are needed for therapeutic applications in which the target cells are notExpand
Cellular dynamics of EGF receptor-targeted synthetic viruses.
The epidermal growth factor receptor (EGFR) is overexpressed on a high percentage of human carcinomas. EGFR is an attractive therapeutic target for tissue-specific targeting by non-viral vectors inExpand
Polycation‐based DNA complexes for tumor‐targeted gene delivery in vivo
Efficient and target‐specific in vivo gene delivery is a major challenge in gene therapy. Compared to cell culture application, in vivo gene delivery faces a variety of additional obstacles such asExpand