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Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent
The characteristics of actinidin (EC 3.4.22.14) and papain (EC 3.4.22.2), two cysteine proteinases whose catalytic-site regions appear to superimpose to a degree that approaches atomic co-ordinateExpand
Cephalosporin Nitrogen Mustard Carbamate prodrugs for “ADEPT”.
A series of cephalosporin carbamates, (6a-d) and (7), functionalised with masked nitrogen mustards has been synthesized. These are potential prodrugs for interaction with a monoclonalExpand
The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.
1. The pH-dependence of the second-order rate constant (k) for the reaction of actinidin (EC 3.4.22.14) with 2-(N'-acetyl-L-phenylalanylamino)ethyl 2'-pyridyl disulphide was determined and theExpand
Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in
1. 2-(N'-Acetyl-L-phenylalanyl)hydroxyethyl 2'-pyridyl disulphide (compound V) was synthesized, and a study of the pH-dependence of the second-order rate constant (k) for its reaction with theExpand
Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.
1. The influence on the reactivities of the catalytic sites of papain (EC 3.4.22.2) and actinidin (3.4.22.14) of providing for interactions involving the S1-S2 intersubsite regions of the enzymes wasExpand
Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding.
1. 2-(N'-Acetyl-L-phenylalanylamino)ethyl 2'-pyridyl disulphide [compound (III)] and 2-(acetamido)ethyl 2'-pyridyl disulphide [compound (IV)] were synthesized by acylation of the common intermediate,Expand
Identification and characterization of chymopapains A and B from dried and fresh latex of Carica papaya
Recent interest in the various cysteine proteinase components of the latex of Carica papaya (Baines & Brocklehurst, 1982; Salih & Brocklehurst, 1983; Buttle & Barrett, 1984; Brocklehurst et al.,Expand
Three-dimensional structure of a B-type chymopapain.
Ct iRIS7'OPHER M. TOPHAM.* JOHN OVERINGTON.* MAIREAD O'DRISCOLL,t# ERDJAN SALIH,tII MARK THOMAS.tll EMRYS W. THOMAS$ and KEITH BROCKLEH URSTt 11 * Liiborii ton of'A4olecirlcrr Biology, Ilep(irttnetiiExpand