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Rat liver cholesterol 7 alpha-hydroxylase. Pretranslational regulation for circadian rhythm.
TLDR
A good correlation of the levels of mRNA to the enzyme activities and the protein levels demonstrates that pretranslational regulation is most likely a mechanism for the circadian rhythm of 7 alpha-hydroxylase. Expand
Cytochrome P450 4A4: expression in Escherichia coli, purification, and characterization of catalytic properties.
Rabbit lung prostaglandin omega-hydroxylase (P450 4A4) was expressed in Escherichia coli using the isopropyl beta-D-thiogalactopyranoside (IPTG) inducible expression vector pCWori+, containing theExpand
Molecular cloning of cDNA for cholesterol 7 alpha-hydroxylase from rat liver microsomes. Nucleotide sequence and expression.
TLDR
The primary structure of cholesterol 7 alpha-hydroxylase deduced from the nucleotide sequence of the cDNA indicated that the enzyme constitutes a novel P-450 family. Expand
Structure of the gene encoding human liver cholesterol 7α-hydroxylase
TLDR
A human cholesterol 7α-hydroxylase gene spanning 17 kb in length which includes the sequence 5′ flanking region and that of the entire transcriptional region was obtained from two partially overlapping clones of a human genomic library. Expand
Structural analysis of the gene encoding rat cholesterol alpha-hydroxylase, the key enzyme for bile acid biosynthesis.
TLDR
Analysis of evolutionary distance between the cholesterol 7 alpha-hydroxylase gene and other known P450 genes indicated that yeast P450LIA is most closely related to P450VIIA. Expand
Mechanism-based inhibitors of prostaglandin omega-hydroxylase: (R)- and (S)-12-hydroxy-16-heptadecynoic acid and 2,2-dimethyl-12-hydroxy-16-heptadecynoic acid.
TLDR
2,2-Dimethyl-12-hydroxy-16-heptadecynoic acid, an analogue that cannot undergo beta-oxidation, has been synthesized as a potential in vivo inhibitor of the enzyme and has been shown to inactivate the purified enzyme with KI = 4.9 microM and t1/2 = 1.0 min. Expand
Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli.
TLDR
Experiments directed toward determining the function of the cytochrome P4504A metabolite, 20-hydroxyarachidonic acid (20-Hydroxyeicosatetraenoic acid; 20-HETE), in cellular ion flux, immunohistochemical localization in lung, and the structure-function determinants which govern the activities of the enzymes encoded by this gene subfamily are reported. Expand
Molecular cloning of cDNA for cholesterol 7α‐hydroxylase from rat liver microsomes
Cholesterol 7α‐hydroxylase; Cytochrome P‐450; cDNA cloning; (COS cell)
Structure of the gene encoding human liver cholesterol 7 alpha-hydroxylase.
TLDR
A human cholesterol 7 alpha-hydroxylase gene spanning 17 kb in length which includes the sequence 5' flanking region and that of the entire transcriptional region and the exon-intron boundaries are completely identical with that of rat gene. Expand