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The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen.
Summary The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition of superoxide dismutase. ThisExpand
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Senescence marker protein 30 functions as gluconolactonase in L-ascorbic acid biosynthesis, and its knockout mice are prone to scurvy.
We originally identified senescence marker protein 30 (SMP30) as a distinctive protein whose expression decreases in an androgen-independent manner with aging. Here, we report its sequence homologyExpand
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Cloning and chromosomal mapping of the human nonfunctional gene for L-gulono-gamma-lactone oxidase, the enzyme for L-ascorbic acid biosynthesis missing in man.
Man is among the exceptional higher animals that are unable to synthesize L-ascorbic acid because of their deficiency in L-gulono-gamma-lactone oxidase, the enzyme catalyzing the terminal step inExpand
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Oxidation of ascorbic acid with superoxide anion generated by the xanthine-xanthine oxidase system.
  • M. Nishikimi
  • Chemistry, Medicine
  • Biochemical and biophysical research…
  • 17 March 1975
Abstract Ascorbic acid was found to be oxidized by O2 • which was generated by the xanthine-xanthine oxidase system. From a kinetic analysis of the inhibition of this reaction by superoxideExpand
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Molecular Cloning and Functional Expression of Rat Liver Glutathione-dependent Dehydroascorbate Reductase*
We have isolated a cDNA clone for a novel glutathione-dependent dehydroascorbate reductase from a rat liver cDNA library in λgt11 by immunoscreening. The authenticity of the clone was confirmed asExpand
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Guinea pigs possess a highly mutated gene for L-gulono-gamma-lactone oxidase, the key enzyme for L-ascorbic acid biosynthesis missing in this species.
Guinea pigs cannot synthesize L-ascorbic acid because of their deficiency in L-gulono-gamma-lactone oxidase, a key enzyme for the biosynthesis of this vitamin in higher animals. In this study weExpand
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Isolation and sequence analysis of a complementary DNA encoding rat liver L-gulono-gamma-lactone oxidase, a key enzyme for L-ascorbic acid biosynthesis.
L-Gulono-gamma-lactone oxidase, one of the microsomal flavin enzymes, catalyzes the last step of L-ascorbic acid biosynthesis in many animals; however, it is missing in scurvy-prone animals such asExpand
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Occurrence in humans and guinea pigs of the gene related to their missing enzyme L-gulono-gamma-lactone oxidase.
Humans, other primates, and guinea pigs are missing an enzyme L-gulono-gamma-lactone oxidase which catalyzes the last step of L-ascorbic acid biosynthesis. We have recently isolated a cDNA encodingExpand
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Purification and characterization of L-gulonolactone oxidase from chicken kidney microsomes.
L-Gulonolactone oxidase was purified from chicken kidney to homogeneity by a five-step method with a recovery of ca. 30%. The molecular weight of the purified enzyme under nondenaturing conditionsExpand
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Occurrence in yeast of L-galactonolactone oxidase which is similar to a key enzyme for ascorbic acid biosynthesis in animals, L-gulonolactone oxidase.
l-Galactonolactone oxidase is believed to catalyze the last step of l-ascorbic acid biosynthesis in yeast. A highly purified preparation of this enzyme from baker's yeast was obtained by a seven-stepExpand
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