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Expression of chimeric receptor composed of immunoglobulin-derived V regions and T-cell receptor-derived C regions.
Chimeric genes composed of immunoglobulin (Ig)-derived variable (V) regions and T-cell receptor (TCR)-derived constant (C) regions were constructed and inserted into an expression vector containing both Ecogpt and neo genes, and transfected into EL4 cells. Expand
Flotillin-1 Regulates IgE Receptor-Mediated Signaling in Rat Basophilic Leukemia (RBL-2H3) Cells
Flotillin-1 is an essential molecule in IgE receptor-mediated mast cell activation, and regulates the kinase activity of Lyn in lipid rafts, which is involved in the process of Lyn activation. Expand
Hormone binding induces rapid proteasome-mediated degradation of thyroid hormone receptors.
  • A. Dace, L. Zhao, +6 authors S. Cheng
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 August 2000
A role of T3 is revealed in modulating the functions of TR by regulating its receptor level via the ubiquitin-proteasome degradation pathway. Expand
Rat basophilic leukemia cells express syntaxin-3 and VAMP-7 in granule membranes.
The expression and the localization of SNARE proteins including VAMP-7 which is insensitive to tetanus toxin are investigated and suggest the involvement ofSNARE proteins in the degranulation of RBL-2H3 cells. Expand
Direct neurite-mast cell communication in vitro occurs via the neuropeptide substance P.
It is illustrated that nerve-mast cell cross-talk can occur in the absence of an intermediary transducing cell and that the neuropeptide substance P, operating via NK-1 receptors, is an important mediator of this communication. Expand
Membrane fusion plays an important role in gene transfection mediated by cationic liposomes
The results suggest that at least two steps are effective for gene transfection mediated by the cationic liposomes with cATIONic cholesterol derivatives, including the endocytosis of the liposome/DNA complex into the target cells and the removal of antisense oligonucleotides (plasmid DNAs) from the complex in the endosomes. Expand
Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative
From the results it was found that the vesicles with moderate diameters were moste effective for gene transfection of plasmid pSV2CAT DNA into the target cell. Expand
Complexin II facilitates exocytotic release in mast cells by enhancing Ca2+ sensitivity of the fusion process
The results suggest that complexin II regulates exocytosis positively by translocating to the plasma membrane and enhancing the Ca2+ sensitivity of fusion machinery, although this translocation to the Plasma membrane is not sufficient to trigger exocyTotic membrane fusion. Expand
Confocal and probe microscopy to study gene transfection mediated by cationic liposomes with a cationic cholesterol derivative.
A novel cationic cholesterol derivative with a hydroxyethyl amino head group (I) has been synthesized and used for liposome-mediated gene transfection and it was found that microtubules were involved in the intracellular dynamics of genetransfection. Expand
Effect of NeuroD2 expression on neuronal differentiation in mouse embryonic stem cells
The results suggest that the expression of NeuroD2 requires an appropriate period of about 3 days to elicit neuronal differentiation in ES cells. Expand