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Comparative genomic and phylogeographic analysis of Mycobacterium leprae
Sixteen interrelated SNP subtypes were defined by genotyping both extant and extinct strains of M. leprae from around the world and showed a strong geographical association that reflects the migration patterns of early humans and trade routes, with the Silk Road linking Europe to China having contributed to the spread of leprosy.
On the origin of leprosy
Using comparative genomics, it is demonstrated that all extant cases of leprosy are attributable to a single clone whose dissemination worldwide can be retraced from analysis of very rare single-nucleotide polymorphisms.
PhageTerm: a tool for fast and accurate determination of phage termini and packaging mechanism using next-generation sequencing data
- Julian R. Garneau, F. Depardieu, L. Fortier, D. Bikard, M. Monot
- BiologyScientific Reports
- 15 August 2017
A theoretical and statistical framework to determine DNA termini and phage packaging mechanisms using NGS data is developed and validated using a set of phages with well-established packaging mechanisms representative of the termini diversity.
The Spore Differentiation Pathway in the Enteric Pathogen Clostridium difficile
The results support the view that the top level of the endosporulation network is conserved in evolution, with the sigma factors acting as the key regulators of the pathway, established some 2.5 billion years ago upon its emergence at the base of the Firmicutes Phylum.
Global transcriptional control by glucose and carbon regulator CcpA in Clostridium difficile
The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria, and controls a complex and extended regulatory network through the modulation of a large set of regulators.
Genome-Wide Analysis of Cell Type-Specific Gene Transcription during Spore Formation in Clostridium difficile
Global analysis of developmental gene expression under the control of these sigma factors revealed deviations from the B. subtilis model regarding the communication between mother cell and forespore in C. difficile and showed that the expression of the σE regulon in the mother cell was not strictly under thecontrol of σF despite the fact that the forespores product SpoIIR was required for the processing of pro-σE.
Reannotation of the genome sequence of Clostridium difficile strain 630.
The functions of more than 500 genes annotated previously with putative functions were reannotated based on updated sequence similarities to proteins whose functions have been recently identified by experimental data from the literature.
Adaptive Strategies and Pathogenesis of Clostridium difficile from In Vivo Transcriptomics
The genome-wide temporal expression of C. difficile 630 genes during the first 38 h of mouse colonization was analyzed to identify genes whose expression is modulated in vivo, suggesting that they may play a role in facilitating the colonization process.
The Key Sigma Factor of Transition Phase, SigH, Controls Sporulation, Metabolism, and Virulence Factor Expression in Clostridium difficile
- L. Saujet, M. Monot, B. Dupuy, O. Soutourina, I. Martin-Verstraete
- BiologyJournal of bacteriology
- 13 May 2011
About 60% of the genes that were differentially expressed between exponential and stationary phases, including genes involved in motility, sporulation, and metabolism, were regulated by SigH, which thus appears to be a key regulator of the transition phase in C. difficile.
Characterization of the SigD Regulon of C. difficile and Its Positive Control of Toxin Production through the Regulation of tcdR
SigD appears to be the first positive regulator of the toxin synthesis in C. difficile, and it is established that SigD positively regulates toxin expression via direct control of tcdR transcription.