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Nitrogen control in bacteria.
TLDR
The ubiquity of the nitrogen regulation systems described in the enterics extend to many other genera, at least in gram-negative organisms are shown and those areas and groups of organisms about which there is much still to learn are identified.
PII Signal Transduction Proteins, Pivotal Players in Microbial Nitrogen Control
TLDR
The history of the PII proteins, their structures and biochemistry, and their distribution and functions in prokaryotes are discussed, and emerging evidence that they may regulate the activity of proteins required for transport of nitrogen compounds into the cell is considered.
The crystal structure of the Escherichia coli AmtB–GlnK complex reveals how GlnK regulates the ammonia channel
TLDR
This crystal structure of PII in a complex with one of its targets reveals physiologically relevant conformations of both AmtB and GlnK.
Membrane sequestration of the signal transduction protein GlnK by the ammonium transporter AmtB
TLDR
The membrane sequestration of GlnK by AmtB represents a novel form of signal transduction in which an integral membrane transport protein functions to link the extracellular ammonium concentration to the intracellular responses to nitrogen status.
The Signal Transduction Protein GlnK Is Required for NifL-Dependent Nitrogen Control of nif Gene Expression in Klebsiella pneumoniae
TLDR
Cloned the K. pneumoniae GlnK gene, studied its expression, and shown that a null mutation in glnK prevents NifL from responding to the absence of fixed nitrogen, i.e., from relieving the inhibition of NifA activity.
The 1.3-Å resolution structure of Nitrosomonas europaea Rh50 and mechanistic implications for NH3 transport by Rhesus family proteins
TLDR
Analysis of the structure of a rare bacterial homologue of human Rh50 proteins strongly argues that all Rh proteins are likely to be homotrimers and that the human erythrocyte proteins RhAG and RhCE/D are unlikely to form heterooligomers as previously proposed.
In a class of its own--the RNA polymerase sigma factor sigma 54 (sigma N).
TLDR
Studies of sigma 54 (sigma N), encoded by rpoN, have demonstrated that this sigma is quite distinct both structurally and functionally from the sigma 70 family and the mode of transcription initiation which it mediates may have more in common with that found in eukaryotes than that which occurs with s Sigma 70 and its relatives.
In a class of its own — the RNA polymerase sigma factor σ;54 (σN)
TLDR
Studies of the Sigma factor σ;54 (σN), encoded by rpoN, have demonstrated that this sigma is quite distinct both structurally and functionally from the �u;70 family and the mode of transcription initiation which it mediates may have more in common with that found in eukaryotes than that which occurs with �o;70 and its relatives.
Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes
TLDR
A global in vivo approach was applied to identify the GlnR regulon of Streptomyces venezuelae, which, unlike many actinomycetes, grows in a diffuse manner that is suitable for physiological studies.
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