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Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals.
The expression of metal-binding proteins or peptides in microorganisms and plants in order to enhance heavy metal accumulation and/or tolerance has great potential. Several different peptides andExpand
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Evaluation of genetically attached histidine affinity tails for purification of lactate dehydrogenase from transgenic tobacco
Abstract Lactate dehydrogenase (Bacillus stearothermophilus) has been expressed in transgenic tobacco. To facilitate purification polyhistidine tails were fused to the 5′-end of the gene. TwoExpand
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Selection of cadmium specific hexapeptides and their expression as OmpA fusion proteins in Escherichia coli.
In searching for novel peptides with affinity for cadmium, the phage display technique was utilized. In the selection procedure, cadmium ions were immobilized on a metal chelating Sepharose gel. TheExpand
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Native and chimeric metal-binding lactate dehydrogenase as detection and protection tools for oxidative stress induced by Fenton's reaction
In the present study, a simple and reliable antioxidant screening technique based on lactate dehydrogenase (LDH) oxidation by Cu2+-mediated Fenton's reaction has successfully been developed.Expand
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Enhanced fructose levels in field grown potato tubers expressing a thermostable glucose isomerase
A thermostable glucose isomerase was expressed in Solanum tuberosum Desirée using the tuber-specific granular-bound starch synthase promoter. The fructose content was substantially increased inExpand
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Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants
Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria andExpand
Procédé d'analyse d'un échantillon de molécules d'immunoglobulines
L'invention concerne des procedes d'analyse d'un echantillon d'immunoglobulines, des peptides apparentes, et des trousses pour realiser ces procedes.