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Fluorescent proteins from nonbioluminescent Anthozoa species
We have cloned six fluorescent proteins homologous to the green fluorescent protein (GFP) from Aequorea victoria. Two of these have spectral characteristics dramatically different from GFP, emitting
Fluorescent proteins and their applications in imaging living cells and tissues.
TLDR
The structure, evolution, and function of GFP-like proteins and their numerous applications for in vivo imaging are focused on, with particular attention to recent techniques.
Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution.
The crystal structure of DsRed, a red fluorescent protein from a corallimorpharian, has been determined at 2.0-A resolution by multiple-wavelength anomalous dispersion and crystallographic
2b-RAD: a simple and flexible method for genome-wide genotyping
We describe 2b-RAD, a streamlined restriction site–associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied
Sequencing and de novo analysis of a coral larval transcriptome using 454 GSFlx
TLDR
The methods described here for deep sequencing of the transcriptome should be widely applicable to generate catalogs of genes and genetic markers in emerging model organisms to facilitate genomics studies in corals and other non-model systems.
Diversity and Evolution of Coral Fluorescent Proteins
TLDR
The results highlight the extent of convergent or parallel evolution of the color diversity in corals, provide the foundation for experimental studies of evolutionary processes that led to color diversification, and enable a comparative analysis of structural determinants of different colors.
Genomic determinants of coral heat tolerance across latitudes
TLDR
An up–to–10-fold increase in odds of survival of coral larvae under heat stress when their parents come from a warmer lower-latitude location is shown.
Amplification of cDNA ends based on template-switching effect and step-out PCR.
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be
GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity.
TLDR
Diverse GFP-like proteins from previously undersampled and completely new sources are described, including hydromedusae and planktonic Copepoda, and a new yellow protein seems to follow exactly the same structural solution to achieving the yellow color of fluorescence as YFP, an engineered yellow-emitting mutant variant of GFP.
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