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Novel functional biodegradable polymer IV: pH-sensitive controlled release of fibroblast growth factor-2 from a poly(gamma-glutamic acid)-sulfonate matrix for tissue engineering.
The acidic pH-sensitive controlled release of fibroblast growth factor-2 from a biodegradable hydrogel without any denaturation of the FGF-2 was successfully performed by a combination of F GF-2 activity and acidic pH, and may be useful for tissue engineering fields such as neovascular treatment for ischemia and inflammation. Expand
Three-dimensional cell culture technique and pathophysiology.
A brief overview on current 3D-tissue fabrication technologies and their biomedical applications is provided and it is shown that physiological human tissue responses can be replicated in the nano/micro-meter ranges. Expand
Multicellular spheroid based on a triple co-culture: A novel 3D model to mimic pancreatic tumor complexity.
This new 3D triple co-culture model closely mimicked the resistance to treatments observed in vivo, resulting in a reduction of cancer cell sensitivity to the anticancer treatment. Expand
Development of vascularized iPSC derived 3D-cardiomyocyte tissues by filtration Layer-by-Layer technique and their application for pharmaceutical assays.
Construction of vascularized 3D-iPSC-CM tissues by a newly-discovered filtration-Layer-by-Layer (LbL) technique is reported, finding that the introduction of normal human cardiac fibroblasts into the iPSC -CM tissues modulated organization and synchronous beating depending on NHCF ratios. Expand
Effects of angiogenic factors and 3D-microenvironments on vascularization within sandwich cultures.
3D-microenvironments controlled at the single layer level showed that sandwich culture between more than 3 fibroblast-layers induced tubule formation, and it was found that not only angiogenic factors, but also the 3D- microenvironments of the endothelial cells played crucial roles in tubules formation in vitro. Expand
Development of In Vitro Drug-Induced Cardiotoxicity Assay by Using Three-Dimensional Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells
The 3D-hiPSC-CT exhibited doxorubicin-sensitive cytotoxicity and hERG channel blocker/isoproterenol-sensitive electrical activity in vitro, indicating its usefulness for drug-induced cardiotoxicity assays or drug screening systems for drug discovery. Expand
The construction of 3D-engineered tissues composed of cells and extracellular matrices by hydrogel template approach.
3D-engineered tissues constructed by the hydrogel template approach may be useful as a new class of soft-tissue engineering technology to substitute a synthetic polymer scaffold to the ECM scaffold produced from the cultured cells. Expand
Preparation of Biodegradable Hollow Nanocapsules by Silica Template Method
Biodegradable hollow nanocapsules were successfully prepared by a combination of layer-by-layer assembly and the silica template method. Ultrathin polymer films were constructed on the surface of t...
Seeing Elastin: A Near-Infrared Zwitterionic Fluorescent Probe for In Vivo Elastin Imaging
Summary Elastic fibers are present in a variety of tissues and are responsible for their resilience. Until now, no optical contrast agent in the near-infrared (NIR) wavelength range of 700–900 nm hasExpand