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Identification and expression of the Escherichia coli fdhD and fdhE genes, which are involved in the formation of respiratory formate dehydrogenase
TLDR
Initial experiments indicate that the region between the two genes seems not to be essential to FDH-PMS activity, which might suggest the participation of fdhE in the synthesis of the selenopolypeptide of FDH -PMS.
Microbial hydrogenases: primary structure, classification, signatures and phylogeny.
TLDR
Analyses of motif compositions indicate that all hydrogenases, except those of class VI, must contain some common motifs probably participating in the formation of hydrogen activation domains and electron transfer domains, and that the 30 hydrogenases have evolved from at least three different ancestors.
Antagonistic effect of nickel on the fermentative growth of Escherichia coli K-12 and comparison of nickel and cobalt toxicity on the aerobic and anaerobic growth.
The facultative anaerobic enterobacterium Escherichia coli requires the activity of nickel-containing hydrogenase for its anaerobic growth. Deficiency of the specific nickel transport system led to a
A second phenazine methosulphate-linked formate dehydrogenase isoenzyme in Escherichia coli.
TLDR
A biochemical and immunological study has revealed a new formate dehydrogenase isoenzyme in Escherichia coli that cross reacts with antibodies raised to FDH-N and possesses a similar polypeptide composition to FDh-N, which seems likely to functions physiologically as a formate: quinone oxidoreductase.
Nucleotide sequence of the fdhE gene involved in respiratory formate dehydrogenase formation in Escherichia coli K-12.
Abstract The gene fdhE of Escherichia coli is 936 bp long and codes for a soluble polypeptide required for the synthesis of the phenazine-methosulfate-linked formate dehydrogenase.
Hydrogenase Mutants of Escherichia coli Defective in Nickel Uptake
Two pathways for H2 metabolism have been identified in Escherichia coli and other members of the family Enterobacteriaceae under anaerobic growth conditions. The first pathway, known as the formate