Primase directs the release of DnaC from DnaB.
- M. Makowska-Grzyska, J. Kaguni
- Biology, ChemistryMolecules and Cells
- 15 January 2010
Dynamic Effects of Cofactors and DNA on the Oligomeric State of Human Mitochondrial DNA Helicase*
- Tawn D. Ziebarth, R. González-Soltero, M. Makowska-Grzyska, R. Núnez-Ramírez, J. Carazo, L. Kaguni
- Biology, ChemistryJournal of Biological Chemistry
- 8 March 2010
It is demonstrated that low salt conditions result in protein aggregation that may cause dissociation of oligomeric structure and it is found that single-stranded DNA binding occurs in the absence of cofactors and renders the mitochondrial DNA helicase more susceptible to proteolytic digestion.
New LIC vectors for production of proteins from genes containing rare codons
- W. Eschenfeldt, M. Makowska-Grzyska, L. Stols, M. Donnelly, R. Jedrzejczak, A. Joachimiak
- BiologyJournal of Structural and Functional Genomics
- 22 September 2013
Higher yields of proteins expressed from genes with rare codons occurred in most cases, biotinylated derivatives enabled detailed automated ligand binding analysis, and multiple proteins introduced by dual LIC cloning were expressed successfully and in near balanced stoichiometry, allowing tandem purification of interacting proteins.
First row divalent transition metal complexes of aryl-appended tris((pyridyl)methyl)amine ligands: syntheses, structures, electrochemistry, and hydroxamate binding properties.
- M. Makowska-Grzyska, E. Szajna, L. Berreau
- ChemistryInorganic Chemistry
- 15 October 2003
Divalent manganese, cobalt, nickel, and zinc complexes of 6-Ph(2)TPA are characterized by X-ray crystallographic characterization and NMR spectra indicate the presence of CH/pi interactions, as an upfield-shifted methyl resonance for a bound acetonitrile molecule is present.
Synthesis, in Vitro Evaluation and Cocrystal Structure of 4-Oxo-[1]benzopyrano[4,3-c]pyrazole Cryptosporidium parvum Inosine 5′-Monophosphate Dehydrogenase (CpIMPDH) Inhibitors
- Zhuming Sun, J. Khan, G. Cuny
- Chemistry, BiologyJournal of Medicinal Chemistry
- 4 December 2014
A SAR study for this compound series resulting in 8k (IC50 = 20 ± 4 nM) is reported and an X-ray crystal structure of CpIMPDH·IMP·8k is presented.
The Dimerization Domain in DapE Enzymes Is required for Catalysis
Structural analysis and molecular dynamics simulations indicate that removal of the dimerization domain increased the flexibility of a conserved active site loop that may provide critical interactions with the substrate.
Sensor Domain of Histidine Kinase KinB of Pseudomonas
- K. Tan, G. Chhor, T. A. Binkowski, R. Jedrzejczak, M. Makowska-Grzyska, A. Joachimiak
- Biology, ChemistryJournal of Biological Chemistry
- 26 February 2014
The first crystal structures of the KinB sensor domain, a dimer in solution, and in the crystal it shows an atypical dimer of a helix-swapped four-helix bundle, which forms a ligand-binding cavity on the dimer interface.
High-throughput protein purification and quality assessment for crystallization.
- Youngchang Kim, G. Babnigg, A. Joachimiak
- BiologyMethods
- 1 September 2011
Optimization of benzoxazole-based inhibitors of Cryptosporidium parvum inosine 5'-monophosphate dehydrogenase.
- S. Gorla, M. Kavitha, G. Cuny
- Biology, ChemistryJournal of Medicinal Chemistry
- 23 May 2013
The structure-activity relationship (SAR) relationship for a series of benzoxazole derivatives with many compounds demonstrating CpIMPDH IC50 values in the nanomolar range and >500-fold selectivity over human IMPDH (hIM PDH).
Mycobacterium tuberculosis IMPDH in Complexes with Substrates, Products and Antitubercular Compounds
- M. Makowska-Grzyska, Youngchang Kim, R. Manganelli
- Biology, ChemistryPLoS ONE
- 6 October 2015
Tuberculosis (TB) remains a worldwide problem and the need for new drugs is increasingly more urgent with the emergence of multidrug- and extensively-drug resistant TB. Inosine 5’-monophosphate…
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