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Loss of C. elegans BBS-7 and BBS-8 protein function results in cilia defects and compromised intraflagellar transport.
It is demonstrated that BBS-7 and Bbs-8 are required for the normal localization/motility of the IFT proteins OSM-5/Polaris andCHE-11, and to a notably lesser extent, CHE-2. Expand
A crucial requirement for Hedgehog signaling in small cell lung cancer
Findings show a crucial cell-intrinsic role for Hedgehog signaling in the development and maintenance of SCLC and identify Hedgehog pathway inhibition as a therapeutic strategy to slow the progression of disease and delay cancer recurrence in individuals with S CLC. Expand
Caught Nek-ing: cilia and centrioles
Results of statistical analysis indicate that the Nek family coevolved with centrioles that function as both microtubule-organizing centers and the basal bodies of cilia, which provides new insights into the cellular roles of the proteins and disease with which some of them have been linked. Expand
NIMA-related kinases defective in murine models of polycystic kidney diseases localize to primary cilia and centrosomes.
It is reported that in a mouse kidney epithelial cell line, mN Nek1 localizes to centrosomes in interphase and remains associated with the mitotic spindle pole during mitosis and Knockdown of mNek8 by siRNA does not affect ciliary assembly. Expand
Cep120 is asymmetrically localized to the daughter centriole and is essential for centriole assembly
Cep120, a protein involved in maintenance of neural progenitor cells, is required for centriole duplication in cycling cells and for centriole amplification in tracheal epithelial cells.
The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation.
It is reported that the FA2 NIMA-related kinase also plays a role in cell cycle progression in Chlamydomonas reinhardtii, the first indication that members of the N IMA family might exert their effects through the regulation of microtubule severing. Expand
A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells.
Mice with mutations in Nek1 or Nek8 have cystic kidneys; therefore, the discovery that a member of this phylogenetic group of Nek proteins is localized to the same sites in Chlamydomonas and kidney epithelial cells suggests that Neks play conserved roles in the coordination of cilia and cell cycle progression. Expand
Centrioles are freed from cilia by severing prior to mitosis
It is shown by live cell imaging and immunofluoresence microscopy that resorbing flagella of Chlamydomonas leave remnants associated with the mother cell wall, and it is postulated that the remnants are the product of severing of doublet microtubules between the basal bodies and the flagellar transition zone, thereby freeing the centrioles to participate in spindle organization. Expand
Supernumerary Centrosomes Nucleate Extra Cilia and Compromise Primary Cilium Signaling
It is found that cells with extra centrioles often formed more than one cilium, had reduced ciliary concentration of Smoothened in response to Sonic hedgehog stimulation, and reduced Shh pathway transcriptional activation. Expand
High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy
The authors use correlative 3D-superresolution, electron microscopy and electron tomography to detail the architecture of distal appendages, to describe the initial stages of appendage assembly and show that appendages of mature centrioles remodel during mitosis. Expand