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T4-Related Bacteriophage LIMEstone Isolates for the Control of Soft Rot on Potato Caused by ‘Dickeya solani’
The bacterium ‘Dickeya solani’, an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato, is controlled using bacteriophage therapy and two closely related and specific phages isolated and characterized are deemed suitable and promising for use in phage therapy.
Identification and sensitive endophytic detection of the fire blight pathogen Erwinia amylovora with 23S ribosomal DNA sequences and the polymerase chain reaction
Two short sequences, situated in the bacterial 23S rDNA gene, were used as primers for the PCR detection of Erwinia amylovora bacteria, and reliable PCR detection in woody host plant tissue was only obtained with PVP/PVPP-treated sample extracts.
Clonal expansion of the Belgian Phytophthora ramorum populations based on new microsatellite markers
Based on the SSR analysis there is no evidence of sexual recombination in the Belgian population of P. ramorum, indicating a relatively recent clonal expansion.
Fast classification of plant-associated bacteria in the Xanthomonas genus
A rapid method for identifying Xanthomonas bacteria was developed, based on specific PCR amplification, which was used to screen X anthomonas-related organisms, newly isolated phytobacteria and bacterial populations in wheat seed extracts.
Streptomyces as a plant's best friend?
Here we discuss the advantages of the majority of this versatile and diverse group of microorganisms for plant health and growth as demonstrated by their contribution to disease-suppressive soils,
A suggested new bacteriophage genus: “Viunalikevirus”
A close examination of the tail spike region in the DNA revealed four distinct tail spike proteins, an arrangement which might lead to the umbrella-like structures of the tails visible on electron micrographs, which set the suggested genus apart from the recently ratified subfamily Tevenvirinae, although a significant evolutionary relationship can be observed.
First Report of 'Candidatus Liberibacter solanacearum' on Carrot in Africa.
To the knowledge, this is the first report of the occurrence of 'Ca. L. solanacearum' in Morocco and Africa, suggesting a wider distribution of the bacterium in carrot crops in the Mediterranean region, including North Africa.
Recognition and detection in seed of the Xanthomonas pathogens that cause cereal leaf streak using rDNA spacer sequences and polymerase chain reaction
PCR specificity was largely determined by one primer site, which is located in a 57-bp sequence present only in the rDNA spacer of DNA homology group I strains, indicating the technique might be useful for detecting pathogens in seed.
Quantitative detection and monitoring of Colletotrichum acutatum in strawberry leaves using real‐time PCR
Real-time PCR assays for Colletotrichum acutatum were developed using primers designed to the ribosomal DNA internal transcribed spacer 1 (rDNA ITS1) and the β-tubulin 2 gene and confirmed that leaf age may play a role in the extent of C. acutatum infection.
Sequence Diversity in the Dickeya fliC Gene: Phylogeny of the Dickeya Genus and TaqMan® PCR for 'D. solani', New Biovar 3 Variant on Potato in Europe
A new Dickeya biovar 3 variant, tentatively named ‘Dickeya solani’, has emerged as a common major threat, in particular in seed potatoes and this molecular tool can support the efforts to control this particular phytopathogen in seed potato certification.