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Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities
Phylum- and class-specific PCR primers were tested for the production of clone libraries and for denaturing gradient gel electrophoresis (DGGE) analysis of complex bacterial communities. Primers wereExpand
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Impact of silver nanoparticle contamination on the genetic diversity of natural bacterial assemblages in estuarine sediments.
Recent advancements in the nanotechnology industry have seen a growing interest in integrating silver nanoparticles (Ag-NPs) into consumer and medical products. To date, there has been littleExpand
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Genetic diversity of marine Synechococcus and co-occurring cyanophage communities: evidence for viral control of phytoplankton.
Unicellular cyanobacteria of the genus Synechococcus are a major component of the picophytoplankton and make a substantial contribution to primary productivity in the oceans. Here we provide evidenceExpand
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Practical considerations for conducting ecotoxicity test methods with manufactured nanomaterials: what have we learnt so far?
This review paper reports the consensus of a technical workshop hosted by the European network, NanoImpactNet (NIN). The workshop aimed to review the collective experience of working at the benchExpand
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Culturing marine bacteria – an essential prerequisite for biodiscovery
The potential for using marine microbes for biodiscovery is severely limited by the lack of laboratory cultures. It is a long‐standing observation that standard microbiological techniques onlyExpand
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Variation in fatty acid composition of Arthrospira (Spirulina) strains
A study of the fatty acid composition was made for 35 Arthrospira strains, concentrating on the most abundant fatty acids, the two polyunsaturated C18 acids, linoleic and γ-linolenic acid, andExpand
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The Taxonomic and Functional Diversity of Microbes at a Temperate Coastal Site: A ‘Multi-Omic’ Study of Seasonal and Diel Temporal Variation
How microbial communities change over time in response to the environment is poorly understood. Previously a six-year time series of 16S rRNA V6 data from the Western English Channel demonstratedExpand
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Arthrospira ('Spirulina') strains from four continents are resolved into only two clusters, based on amplified ribosomal DNA restriction analysis of the internally transcribed spacer.
We present the results of a phylogenetic study, based on amplified ribosomal DNA restriction analysis of the rDNA operon, of 37 Arthrospira ('Spirulina') cultivated clonal strains from fourExpand
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A survey of the morphological characters of 36 clonal axenic strains of Arthrospira showed that 34 had helical and 2 had straight trichomes. Of those with helical trichomes, five were right‐handedExpand
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Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms
The impact of ocean acidification and carbonation on microbial community structure was assessed during a large-scale in situ costal pelagic mesocosm study, included as part of the EPOCA 2010 ArcticExpand
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