Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria.
- A. Steinbüchel, E. Hustede, M. Liebergesell, U. Pieper, A. Timm, H. Valentin
- Biology, EngineeringFEMS Microbiology Reviews
- 1 December 1992
Molecular data will be shown for genes of Alcaligenes eutrophus, purple non-sulfur bacteria, Such as Rhodospirillum rubrum, purple sulfur bacteria, such as Chromatium vinosum, pseudomonads belonging to rRNA homology group I, and for the Gram-positive bacterium Rhodococcus ruber.
Considerations on the structure and biochemistry of bacterial polyhydroxyalkanoic acid inclusions.
Some mathematical calculations were done that provided information about the structure and biochemistry of polyhydroxyalkanoic acid (PHA) granules and about the amounts of the different constituents…
Purification and characterization of the poly(hydroxyalkanoic acid) synthase from Chromatium vinosum and localization of the enzyme at the surface of poly(hydroxyalkanoic acid) granules.
- M. Liebergesell, K. Sonomoto, M. Madkour, F. Mayer, A. Steinbüchel
- Biology, EngineeringEuropean Journal of Biochemistry
- 1 November 1994
Immunoblots with anti-PhaC, anti- PhaE IgG and crude extract proteins indicated that poly(3-hydroxyalkanoic acid) synthases with partial sequence similarities are widespread among purple sulphur bacteria.
Cloning and nucleotide sequences of genes relevant for biosynthesis of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D.
From a genomic library of Chromatium vinosum strain D in lambda L47, a 16.5-kbp EcoRI-restriction fragment was identified by hybridization with a DNA fragment harboring the operon for Alcaligenes eutrophus poly(3-hydroxyalkanoate) (PHA) synthesis, which restored the ability to synthesize and accumulate PHA in PHA-negative mutants of A. eUTrophus.
Electron microscopic observations on the macromolecular organization of the boundary layer of bacterial PHA inclusion bodies
The boundary layer of PHA inclusion bodies isolated from Chromatium vinosum, Pseudomonas oleovorans, and Rhodococcus ruber was investigated and revealed that the boundary layer is a monolayer exhibiting only one kind of basic lattice.
Isolation and identification of granule-associated proteins relevant for poly(3-hydroxyalkanoic acid) biosynthesis in Chromatium vinosum D.
Poly(3-hydroxybutyric acid) granules, which harbored only four major granule-associated proteins as revealed by SDS polyacrylamide gel electrophoresis, were isolated from crude cellular extracts of…
Formation of poly(3-hydroxyalkanoates) by phototrophic and chemolithotrophic bacteria
Among 15 strains tested, only Chromatium vinosum strain 1611, C. purpuratum strain BN5500 and Lamprocystis roseopersicina strain 3112 were able to synthesize polyesters containing 3HV with propionate, valerate or heptanoate as carbon source.
Isolation of poly(beta-L-malic acid)-degrading bacteria and purification and characterization of the PMA hydrolase from Comamonas acidovorans strain 7789.
Several bacteria were isolated which were able to utilize poly(beta-L-malic acid) as sole carbon source for growth and hydrolyzing enzyme of Comamonas acidovorans strain 7789 was detected in the membrane fraction.
THE PHOTOPHOBIC RESPONSE OF VARIOUS SULFUR AND NONSULFUR PURPLE BACTERIA
The photophobic response of 34 strains of nonsulfur and sulfur purple bacteria was examined with respect to response‐eliciting light intensities and strains of Rhodospirillum rubrum and Chromatium vinosum were recommended as model organisms for further studies.