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A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression
OTTIP RNA binds the adaptor protein WDR5 directly and targets WDR 5/MLL complexes across HOXA, driving histone H3 lysine 4 trimethylation and gene transcription.
The POT1–TPP1 telomere complex is a telomerase processivity factor
The crystal structure of a domain of human TPP1 reveals an oligonucleotide/oligosaccharide-binding fold that is structurally similar to the β-subunit of the telomere end-binding protein of a ciliated protozoan, suggesting that TPP1 is the missing β- subunit of human POT1 protein.
Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection
The crystal structure of the N-terminal half of human POT1 (hPOT1) protein bound to a telomeric single-stranded DNA (ssDNA) decamer, TTAGGGTTAG, is reported, providing an atomic-resolution model for chromosome end-capping.
Crystal structures of c-Src reveal features of its autoinhibitory mechanism.
Four additional c-Src structures in which this segment adopts an ordered but inhibitory conformation are reported, in which the ordered activation loop forms an alpha helix that stabilizes the inactive conformation of the kinase domain, blocks the peptide substrate-binding site, and prevents Tyr-416 phosphorylation.
Arsenic degrades PML or PML–RARα through a SUMO-triggered RNF4/ubiquitin-mediated pathway
PML is identified as the first protein degraded by SUMO-dependent polyubiquitination, as it recruits not only RNF4, ubiquitin and proteasomes, but also many SUMOylated proteins onto PML nuclear bodies, which could physically integrate thesumOylation, ubiquItination and degradation pathways.
Structure of PAK1 in an Autoinhibited Conformation Reveals a Multistage Activation Switch
The p21-activated kinases, stimulated by binding with GTP-liganded forms of Cdc42 or Rac, modulate cytoskeletal actin assembly and activate MAP-kinase pathways and a related switch has been seen in the Wiskott-Aldrich syndrome protein (WASP).
A Shared Docking Motif in TRF1 and TRF2 Used for Differential Recruitment of Telomeric Proteins
This work dissects the interactions of TRF1 and TRF2 with their shared binding partner (TIN2) and other shelterin accessory factors, including Apollo, which interacts with another shelterin-associated factor: PinX1.
Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis.
DBF4, which encodes the regulatory subunit of a Cdk-like protein kinase Cdc7-Dbf4, is identified in a screen for second site suppressors of mcm2-1 and data suggest that phosphorylation of Mcm2 and probably other members of the Mcm 2-7 proteins by CDC7- Dbf4 at the G1-to-S phase transition is a critical step in the initiation of DNA synthesis at replication origins.
Pak1 kinase homodimers are autoinhibited in trans and dissociated upon activation by Cdc42 and Rac1.
The crystal structure of Pak1 revealed an autoinhibited dimer that must dissociate upon GTPase binding, and it is shown that Pak1 forms homodimers in vivo and that its dimerization is regulated by the intracellular level of GTP-Cdc42 orGTP-Rac1.
Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases.
The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl