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Stoichiometry and Architecture of Active DNA Replication Machinery in Escherichia coli
Forking Replisomes Replisomes are multiprotein machines that replicate DNA. Significant insight into how they work comes from in vitro studies, but how replisomes are organized in living cells hasExpand
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Stoichiometry and turnover in single, functioning membrane protein complexes
Many essential cellular processes are carried out by complex biological machines located in the cell membrane. The bacterial flagellar motor is a large membrane-spanning protein complex thatExpand
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The maximum number of torque-generating units in the flagellar motor of Escherichia coli is at least 11.
Torque is generated in the rotary motor at the base of the bacterial flagellum by ion translocating stator units anchored to the peptidoglycan cell wall. Stator units are composed of the proteinsExpand
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Passive Stiffness Changes Caused by Upregulation of Compliant Titin Isoforms in Human Dilated Cardiomyopathy Hearts
In the pathogenesis of dilated cardiomyopathy, cytoskeletal proteins play an important role. In this study, we analyzed titin expression in left ventricles of 19 control human donors and 9 severelyExpand
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Variable stoichiometry of the TatA component of the twin-arginine protein transport system observed by in vivo single-molecule imaging
The twin-arginine translocation (Tat) system transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membrane of plant chloroplasts. The essential components of the TatExpand
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Direct observation of steps in rotation of the bacterial flagellar motor
The bacterial flagellar motor is a rotary molecular machine that rotates the helical filaments that propel many species of swimming bacteria. The rotor is a set of rings up to 45 nm in diameter inExpand
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Association of the Chaperone αB-crystallin with Titin in Heart Muscle*
αB-crystallin, a major component of the vertebrate lens, is a chaperone belonging to the family of small heat shock proteins. These proteins form oligomers that bind to partially unfolded substratesExpand
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Developmentally Regulated Switching of Titin Size Alters Myofibrillar Stiffness in the Perinatal Heart
Abstract— Before birth, the compliance of the heart is limited predominantly by extracardiac constraint. Reduction of this constraint at birth requires that myocardial compliance be determined mainlyExpand
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In Vivo Architecture and Action of Bacterial Structural Maintenance of Chromosome Proteins
Making a Move Structural Maintenance of Chromosome (SMC) complexes act ubiquitously in chromosome processing in all domains of life, but their mode of action in living cells has remained an enigma.Expand
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The elasticity of single titin molecules using a two-bead optical tweezers assay.
Titin is responsible for the passive elasticity of the muscle sarcomere. The mechanical properties of skeletal and cardiac muscle titin were characterized in single molecules using a novel dualExpand
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