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The Protein Interaction Network of the Human Transcription Machinery Reveals a Role for the Conserved GTPase RPAP4/GPN1 and Microtubule Assembly in Nuclear Import and Biogenesis of RNA Polymerase II*
RPAP4/GPN1 is a member of a newly discovered GTPase family that contains a unique and highly conserved GPN loop motif that is essential, in conjunction with its GTP-binding motifs, for nuclear localization of POLR2A/RPB1 in a process that also requires microtubule assembly.
A Newly Uncovered Group of Distantly Related Lysine Methyltransferases Preferentially Interact with Molecular Chaperones to Regulate Their Activity
- Philippe Cloutier, M. Lavallée-Adam, D. Faubert, M. Blanchette, B. Coulombe
- BiologyPLoS genetics
- 1 January 2013
A new role for protein methylation as a regulatory pathway for molecular chaperones is uncovered and a novel regulatory mechanism for the chaperone VCP, whose deregulation is causative of degenerative neuromuscular diseases is defined.
ΔF508 CFTR interactome remodeling promotes rescue of Cystic Fibrosis
It is demonstrated that global remodelling of ∆F508 CFTR interactions is crucial for rescue, and comprehensive insight is provided into the molecular disease mechanisms of cystic fibrosis caused by deletion of F508.
Recessive mutations in POLR1C cause a leukodystrophy by impairing biogenesis of RNA polymerase III
This study is the first to show that distinct mutations in a gene coding for a shared subunit of two RNA polymerases lead to selective modification of the enzymes' availability leading to two different clinical conditions and to shed some light on the pathophysiological mechanism of one of the most common hypomyelinating leukodystrophies, POLR3-related leukODYstrophy.
A complex of C9ORF72 and p62 uses arginine methylation to eliminate stress granules by autophagy
It is demonstrated that C9ORF72 and the autophagy receptor p62 interact to associate with proteins symmetrically dimethylated on arginines such as FUS, to eliminate stress granules by autophagic.
The Sorting Receptor SorCS1 Regulates Trafficking of Neurexin and AMPA Receptors
High-resolution mapping of the protein interaction network for the human transcription machinery and affinity purification of RNA polymerase II-associated complexes.
Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2
- D. Forget, Andrée-Anne Lacombe, Philippe Cloutier, M. Lavallée-Adam, M. Blanchette, B. Coulombe
- BiologyNucleic acids research
- 30 May 2013
The results have important implications, as they indicate that RPAP2 controls gene expression by two distinct mechanisms, one that targetsRNAP II activity during transcription and the other that controls availability of RNAP II in the nucleus.
PSEA-Quant: A Protein Set Enrichment Analysis on Label-Free and Label-Based Protein Quantification Data
- M. Lavallée-Adam, N. Rauniyar, Daniel B. McClatchy, J. Yates
- BiologyJournal of proteome research
- 1 September 2014
PSEA-Quant, a protein set enrichment analysis algorithm for label-free and label-based protein quantification data sets, offers an alternative approach to classic GO analyses, models protein annotation biases, and allows the analysis of samples originating from a single condition, unlike analogous approaches such as GSEA and PSEA.
Quantitative Proteomics of Human Fibroblasts with I1061T Mutation in Niemann–Pick C1 (NPC1) Protein Provides Insights into the Disease Pathogenesis*
- N. Rauniyar, Kanagaraj Subramanian, M. Lavallée-Adam, S. Martínez-Bartolomé, W. Balch, J. Yates
- BiologyMolecular & Cellular Proteomics
- 14 April 2015
This study observed that treating NPC1I1061T cells with four classes of seven different compounds that are potential NPC drugs increased the expression level of SOD2 and DHCR24, and shown an abnormal accumulation of glycogen in NPCs fibroblasts possibly triggered by defective processing of lysosomal alpha-glucosidase.