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Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier.
TLDR
Insertional mutants unable to synthesize or translocate DIMs exhibit higher cell wall permeability and are more sensitive to detergent than the wild type strain, indicating for the first time that, in addition to being important virulence factors, extractable lipids of M. tuberculosis play a role in the cell envelope architecture and permeability. Expand
Role of the pks15/1 gene in the biosynthesis of phenolglycolipids in the Mycobacterium tuberculosis complex. Evidence that all strains synthesize glycosylated p-hydroxybenzoic methyl esters and that
TLDR
Data indicate that Pks15/1 is involved in the elongation of p-hydroxybenzoic acid to give p-Hydroxyphenylalkanoates, which in turn are converted, presumably by the PpsA-E synthase, to phenolphthiocerol derivatives. Expand
Structure of the cell envelope of corynebacteria: importance of the non-covalently bound lipids in the formation of the cell wall permeability barrier and fracture plane.
TLDR
A combination of molecular compositional analysis, ultrastructural appearance and freeze-etch electron microscopy study was used to arrive at a chemical model, unique to corynebacteria but consistent with their phylogenetic relatedness to mycobacteria and other members of the distinctive suprageneric actinomycete taxon. Expand
Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice
TLDR
A mutant strain of M. tuberculosis with an inactivated hma (cmaA, mma4) gene is constructed; this mutant strain no longer synthesizes oxygenated mycolic acids, has profound alterations in its envelope permeability and is attenuated in mice. Expand
Mycolic acids: structures, biosynthesis, and beyond.
TLDR
Progress in deciphering the mycolic acid biosynthetic pathway is described and the functional and key biological roles of these molecules are discussed, providing a historical perspective in this dynamic area. Expand
Inactivation of the antigen 85C gene profoundly affects the mycolate content and alters the permeability of the Mycobacterium tuberculosis cell envelope
TLDR
Data demonstrate that: (i) antigen 85C is involved directly or indirectly in the transfer of mycolates onto the cell wall of the whole bacterium; (ii) the enzyme is not specific for a given type of my colate; and (iii) the cellwall‐linked mycolate layer may represent a barrier for the diffusion of small hydrophobic and hydrophilic molecules. Expand
The Acyl-AMP Ligase FadD32 and AccD4-containing Acyl-CoA Carboxylase Are Required for the Synthesis of Mycolic Acids and Essential for Mycobacterial Growth
TLDR
Comp comparative genomics and applied a combination of molecular biology and proteomic technologies to the analysis of proteins that co-immunoprecipitated with AccD4 resulted in the identification of AccA3 and AccD5 as subunits of the acyl-CoA carboxylase. Expand
Identification of the surface-exposed lipids on the cell envelopes of Mycobacterium tuberculosis and other mycobacterial species
TLDR
Analysis of the exposed lipids demonstrated a selective location of classes of ubiquitous lipids on the surfaces of mycobacteria, leading to the proposal of a scheme for the location of the capsular lipids of the tubercle bacillus. Expand
Extracellular enzyme activities potentially involved in the pathogenicity of Mycobacterium tuberculosis.
TLDR
The presence of the eight enzyme activities in the outermost compartments of obligate pathogens and their absence in those of non-pathogens provides further evidence that these enzymes may be involved in the pathogenicity of mycobacteria. Expand
Ornithine lipid of Mycobacterium tuberculosis: its distribution in some slow- and fast-growing mycobacteria.
TLDR
An ornithine-amide lipid is present in Mycobacterium tuberculosis and its structure was established by a combination of chemical analysis and mass spectrometry, revising an earlier reported structure for the lipid from M. bovis. Expand
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