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Characterization of a Kunitz trypsin inhibitor with a single disulfide bridge from seeds of Inga laurina (SW.) Willd.
ILTI was found to be a single polypeptide chain containing 180 amino acids, the sequence of which was clearly homologous to the Kunitz family of serine protease plant protein inhibitors, and it also showed significant similarity to the seed storage proteins, sporamin and miraculin. Expand
A Kunitz-type inhibitor of coleopteran proteases, isolated from Adenanthera pavonina L. seeds and its effect on Callosobruchus maculatus.
A trypsin inhibitor (ApTI) isolated from Adenanthera pavonina seeds showed activity against papain and was highly effective against digestive proteinases from C. maculatus, Acanthoscelides obtectus (bean weevil), and Zabrotes subfasciatus (Mexican bean weevil). Expand
Understanding bacterial resistance to antimicrobial peptides: From the surface to deep inside.
Multiple strategies by which bacteria could develop enhanced antimicrobial peptide resistance are discussed, focusing on sub-cellular regions from the surface to deep inside, evaluating bacterial membranes, cell walls and cytoplasmic metabolism. Expand
Trypsin inhibitor from Dimorphandra mollis seeds: purification and properties.
A trypsin inhibitor from Dimorphandra mollis seeds was isolated to apparent homogeneity by a combination of ammonium sulfate precipitation, gel filtration, ion-exchange and affinity chromatographicExpand
Purification and Characterization of a New Trypsin Inhibitor from Dimorphandra mollis Seeds
A second trypsin inhibitor (DMTI-II) was purified from the seed of Dimorphandra mollis by ammonium sulfate precipitation, gel filtration, and ion-exchange and affinity chromatography, and showed a high degree of homology with other Kunitz-type inhibitors. Expand
Poor correlation between the levels of proteinase inhibitors found in seeds of different cultivars of cowpea (Vigna unguiculata) and the resistance/susceptibility to predation by Callosobruchus
The C. maculatus resistant cowpea seeds support the development of Zabrotes subfasciatus, a bruchid that infests seeds of both Phaseolus vulgaris and Vigna unguiculata. Expand
Isolation and partial characterization of a novel lectin from Talisia esculenta seeds that interferes with fungal growth
A novel plant lectin has been isolated from the seeds of Talisia esculenta and partially characterized and a search of sequence databases showed that the N-terminal sequence had no homology to any known protein. Expand
Vicilin variants and the resistance of cowpea (Vigna unguiculata) seeds to the cowpea weevil (Callosobruchus maculatus)
The results strongly suggest that the resistance of cowpea seeds from the cultivar TVu 2027 and from others bred from it is associated with the presence of vicilin molecules which are refractory to digestion by bruchid midgut proteinases. Expand
Purification and characterization of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effect on the larval development of Callosobruchus maculatus (Coleoptera:
The transformation of genes coding for this lectin could be useful in the development of insect resistance in important agricultural crops. Expand
Evaluation of seed coagulant Moringa oleifera lectin (cMoL) as a bioinsecticidal tool with potential for the control of insects
The lectin presented a tight binding to a chitin column, suggesting that the insecticidal activity of cMoL involves carbohydrate-lectin interactions on the surface of the digestive tract, with glycoproteins and others glycolsylated structures in the midgut and resistance to enzymatic digestion. Expand