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The two analogous phosphoglycerate mutases of Escherichia coli
TLDR
Over‐expressed, purified and characterised the two PGMs of Escherichia coli, finding that dPGM has a 10‐fold higher specific activity than iPGM, and the presence of an integral manganese ion iniPGM was confirmed by EPR spectroscopy. Expand
Structural basis for high-affinity binding of LEDGF PWWP to mononucleosomes
TLDR
It is concluded that cooperative binding of the hydrophobic cavity and basic surface to the cognate histone peptide and DNA wrapped in MNs is essential for high-affinity binding to chromatin. Expand
Interactions of HIV-1 Gag with assembly cofactors.
TLDR
It is suggested that concerted binding of PI(4,5)P2 and nucleic acid to the matrix and nucleocapsid domains, respectively, promotes protein-protein interactions involving capsid domains that must be involved in virus particle assembly. Expand
Multimode, Cooperative Mechanism of Action of Allosteric HIV-1 Integrase Inhibitors*
TLDR
2-(quinolin-3-yl) acetic acid derivatives impairs both integrase-LEDGF binding and LEDGF-independent integrase catalytic activities with similar IC50 values, defining them as bona fide allosteric inhibitors of integrase function. Expand
BET proteins promote efficient murine leukemia virus integration at transcription start sites
TLDR
It is shown that purified recombinant Brd4(1-720) binds with high affinity to MLV integrase and stimulates correct concerted integration in vitro and elucidate the importance of BET proteins for MLV integration efficiency and targeting and provide a route to developing safer MLV-based vectors for human gene therapy. Expand
Interactions between HIV-1 Gag molecules in solution: an inositol phosphate-mediated switch.
TLDR
The participation of both ends of Gag in IP6 interaction suggests that Gag is folded over in solution, with its ends near each other in three-dimensional space, which implies that it undergoes a major conformational change during particle assembly. Expand
Allosteric integrase inhibitor potency is determined through the inhibition of HIV-1 particle maturation
TLDR
It is demonstrated that ALLinI potency is unexpectedly accounted for during the late phase of HIV-1 replication, and cooperative multimerization of IN by ALLINIs together with the inability for LEDGF/p75 to effectively engage the virus during its egress from cells underscores the multimodal mechanism of ALLINI action. Expand
Purification and Characterization of a Novel Class III Peroxidase Isoenzyme from Tea Leaves
TLDR
A novel, basic (isoelectric point > 10), heme peroxidase isoenzyme (TP) that can account for a significant part of the ascorbate per oxidase activity in tea (Camellia sinensis) leaves has been purified to homogeneity and is shown to be the first example, to the authors' knowledge, of a class III peroxIDase with a high specificity for asCorbate as an electron donor. Expand
Cryo-EM structures and atomic model of the HIV-1 strand transfer complex intasome
TLDR
Cryo–electron microscopy reveals how lentiviral DNA and the viral integrase assemble to promote retroviral integration into host cell DNA and helps resolve previous conflicting models of intasome assembly. Expand
Pre-existing Distortions in Nucleic Acid Structure Aid Polypurine Tract Selection by HIV-1 Reverse Transcriptase*
TLDR
The data collectively suggest that the entire PPT sequence contributes to the structural distortion at the PPT/U3 junction, potentially providing a mechanism for its selective processing. Expand
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