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Organelle pH studies using targeted avidin and fluorescein-biotin.
Biochemical Characterization of CA IX, One of the Most Active Carbonic Anhydrase Isozymes*
- M. Hilvo, Lina Baranauskienė, S. Parkkila
- Biology, ChemistryJournal of Biological Chemistry
- 10 October 2008
Investigations on the oligomerization and stability of the enzymes revealed that both recombinant proteins form dimers that are stabilized by intermolecular disulfide bond(s).
Isolation and Characterization of Novosphingobium sp. Strain MT1, a Dominant Polychlorophenol-Degrading Strain in a Groundwater Bioremediation System
- M. Tiirola, M. Männistö, J. Puhakka, M. Kulomaa
- Biology, EngineeringApplied and Environmental Microbiology
- 1 January 2002
The molecular study revealed that the process was dependent on a stable bacterial community with low species diversity, and Phenotypic dimorphism, low growth rate, and low-copy-number 16S rDNA genes were characteristic of strain MT1 and other MT1-like organisms isolated from the bioreactor.
Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family.
Biotin dissociation and ITC (isothermal titration calorimetry) experiments showed dimeric rhizavidin to be a high-affinity biotin-binding protein, lower than that of avidin; although similar to streptavidin, it was insensitive to proteinase K.
Baculovirus capsid display: a novel tool for transduction imaging.
Genetically engineered avidins and streptavidins
- O. Laitinen, V. Hytönen, H. Nordlund, M. Kulomaa
- Biology, ChemistryCellular and Molecular Life Sciences CMLS
- 3 November 2006
The genetically engineered (strept)avidins are summarized, summarizing how these constructs were designed and how they have improved the authors' understanding of the structural and functional characteristics of these proteins, and the benefits they have provided for (strePT)avidin-biotin technology.
Evidence for Natural Horizontal Transfer of the pcpB Gene in the Evolution of Polychlorophenol-Degrading Sphingomonads
- M. Tiirola, Hong Wang, L. Paulin, M. Kulomaa
- BiologyApplied and Environmental Microbiology
- 1 September 2002
A comparative analysis of the 16S rDNA and pcpB gene trees suggested that a recent horizontal transfer of the pCPB gene was involved in the evolution of the catabolic pathway in the Kärkölä sphingomonads.
Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin.
Differences were found in some physico-chemical properties of the AVRs as compared with avidin, including lowered pI, increased glycosylation and, most notably, reversible biotin binding for two AVs (AVR1 and AVR2), making the Avrs applicable for improved avidin-biotin technological applications.
Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery
Baculoviruses are able to mediate transient gene transfer in vivo and may become useful tools for gene therapy by constructing a bacULovirus bearing a nuclear targeted β-galactosidase marker gene (LacZ) under a CMV promoter.