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Organelle pH studies using targeted avidin and fluorescein-biotin.
BACKGROUND Mammalian organelles of the secretory pathway are of differing pH. The pH values form a decreasing gradient: the endoplasmic reticulum (ER) is nearly neutral, the Golgi is mildly acidicExpand
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Biochemical Characterization of CA IX, One of the Most Active Carbonic Anhydrase Isozymes*
Carbonic anhydrase IX (CA IX) is an exceptional member of the CA protein family; in addition to its classical role in pH regulation, it has also been proposed to participate in cell proliferation,Expand
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Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family.
Rhizobium etli CFN42 is a symbiotic nitrogen-fixing bacterium of the common bean Phaseolus vulgaris. The symbiotic plasmid p42d of R. etli comprises a gene encoding a putative (strept)avidin-likeExpand
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Baculovirus capsid display: a novel tool for transduction imaging.
Baculoviruses are enveloped insect viruses that can carry large quantities of foreign DNA in their genome. Baculoviruses have proved to be very promising gene therapy vectors but little is knownExpand
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Evidence for Natural Horizontal Transfer of the pcpB Gene in the Evolution of Polychlorophenol-Degrading Sphingomonads
ABSTRACT The chlorophenol degradation pathway in Sphingobium chlorophenolicum is initiated by the pcpB gene product, pentachlorophenol-4-monooxygenase. The distribution of the gene was studied in aExpand
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Genetically engineered avidins and streptavidins
Abstract.Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques toExpand
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Isolation and Characterization of Novosphingobium sp. Strain MT1, a Dominant Polychlorophenol-Degrading Strain in a Groundwater Bioremediation System
ABSTRACT A high-rate fluidized-bed bioreactor has been treating polychlorophenol-contaminated groundwater in southern Finland at 5 to 8°C for over 6 years. We examined the microbial diversity of theExpand
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Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin.
Chicken avidin and bacterial streptavidin are proteins familiar from their use in various (strept)avidin-biotin technological applications. Avidin binds the vitamin biotin with the highest affinityExpand
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Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery
Recombinant Autographa californica multiple nuclear polyhedrosis viruses (AcMNPV) have recently been shown to transduce mammalian cells in vitro. Since baculoviruses offer many advantages overExpand
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Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin‐like domain
Sea urchin fibropellins are epidermal growth factor homologues that harbor a C‐terminal domain, similar in sequence to hen egg‐white avidin and bacterial streptavidin. The fibropellin sequence wasExpand
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