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MicroRNAs expressed by herpes simplex virus 1 during latent infection regulate viral mRNAs
Herpesviruses are characterized by their ability to maintain life-long latent infections in their animal hosts. However, the mechanisms that allow establishment and maintenance of the latent stateExpand
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Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
This unit describes a method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of the sequenceExpand
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Numerous Conserved and Divergent MicroRNAs Expressed by Herpes Simplex Viruses 1 and 2
ABSTRACT Certain viruses use microRNAs (miRNAs) to regulate the expression of their own genes, host genes, or both. Previous studies have identified a limited number of miRNAs expressed by herpesExpand
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Prediction and Identification of Herpes Simplex Virus 1-Encoded MicroRNAs
ABSTRACT MicroRNAs (miRNAs) are key regulators of gene expression in higher eukaryotes. Recently, miRNAs have been identified from viruses with double-stranded DNA genomes. To attempt to identifyExpand
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High‐Throughput Real‐Time Quantitative Reverse Transcription PCR
Extensive detail on the application of the real‐time quantitative polymerase chain reaction (QPCR) for the analysis of gene expression is provided in this unit. The protocols are designed forExpand
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Stem‐Loop RT‐qPCR for miRNAs
  • M. Kramer
  • Biology, Medicine
  • Current protocols in molecular biology
  • 1 July 2011
This unit presents a specific and sensitive quantitative reverse‐transcription PCR (RT‐qPCR) method for measuring individual microRNAs (miRNAs) in tissue or cultured cells. miRNAs are 17 to 24Expand
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Enzymatic amplification of DNA by PCR: standard procedures and optimization.
  • M. Kramer, D. Coen
  • Chemistry, Biology
  • Current protocols in molecular biology
  • 1 October 2001
This unit describes a method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of the sequenceExpand
  • 44
  • 11
Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
  • M. Kramer, D. Coen
  • Chemistry, Medicine
  • Current protocols in cell biology
  • 1 April 2001
This appendix describes a method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of theExpand
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Quantification of transcripts from the ICP4 and thymidine kinase genes in mouse ganglia latently infected with herpes simplex virus.
Herpes simplex virus establishes latency in nervous tissue in which it is maintained for the life of the mammalian host, with occasional reactivation leading to subsequent spread. Latency-associatedExpand
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Importance of the herpes simplex virus UL24 gene for productive ganglionic infection in mice.
The UL24 gene of herpes simplex virus overlaps the viral thymidine kinase (tk) gene. Most previous studies of UL24 have examined UL24 mutants that have also contained tk and sometimes otherExpand
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