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Strain improvement of recombinant Escherichia coli for efficient production of plant flavonoids.
Plant flavonoid polyphenols continue to find increasing pharmaceutical and nutraceutical applications; however their isolation, especially of pure compounds, from plant material remains an underlyingExpand
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Engineering Central Metabolic Pathways for High-Level Flavonoid Production in Escherichia coli
ABSTRACT The identification of optimal genotypes that result in improved production of recombinant metabolites remains an engineering conundrum. In the present work, various strategies to reengineerExpand
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High-Yield Resveratrol Production in Engineered Escherichia coli †
ABSTRACT Plant polyphenols have been the subject of several recent scientific investigations since many of the molecules in this class have been found to be highly active in the human body, with aExpand
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Increased Malonyl Coenzyme A Biosynthesis by Tuning the Escherichia coli Metabolic Network and Its Application to Flavanone Production
ABSTRACT Identification of genetic targets able to bring about changes to the metabolite profiles of microorganisms continues to be a challenging task. We have independently developed a cipher ofExpand
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Genome-scale metabolic network modeling results in minimal interventions that cooperatively force carbon flux towards malonyl-CoA.
Malonyl-coenzyme A is an important precursor metabolite for the biosynthesis of polyketides, flavonoids and biofuels. However, malonyl-CoA naturally synthesized in microorganisms is consumed for theExpand
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Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli.
Flavonols are plant polyphenolic compounds that belong to the class of molecules collectively known as flavonoids. Because of their demonstrated health benefits towards a wide array of humanExpand
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High‐yield anthocyanin biosynthesis in engineered Escherichia coli
Anthocyanins are red, purple, or blue plant water‐soluble pigments. In the past two decades, anthocyanins have received extensive studies for their anti‐oxidative, anti‐inflammatory, anti‐cancer,Expand
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Modular optimization of multi-gene pathways for fatty acids production in E. coli.
Microbial fatty acid-derived fuels have emerged as promising alternatives to petroleum-based transportation fuels. Here we report a modular engineering approach that systematically removed metabolicExpand
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Biosynthesis of Natural Flavanones in Saccharomyces cerevisiae
ABSTRACT A four-step flavanone biosynthetic pathway was constructed and introduced into Saccharomyces cerevisiae. The recombinant yeast strain was fed with phenylpropanoid acids and produced theExpand
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CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli.
Programmable control over an addressable global regulator would enable simultaneous repression of multiple genes and would have tremendous impact on the field of synthetic biology. It has recentlyExpand
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