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Anticancer Drugs, Ionophoric Peptides, and Steroids as Substrates of the Yeast Multidrug Transporter Pdr5p*
- M. Kołaczkowski, van der Rest Michel, A. Cybularz-Kolaczkowska, J. Soumillion, W. Konings, Goffeau André
- Biology, ChemistryJournal of Biological Chemistry
- 6 December 1996
It is shown that in spite of little sequence homology, and inverted topology in respect to that of mammalian P-glycoproteins, Pdr5p shares with them common substrates, and the experimental system opens new possibilities for the analysis of structure-function relationship of multidrug transporter substrates and inhibitors.
The pleitropic drug ABC transporters from Saccharomyces cerevisiae.
- B. Rogers, A. Decottignies, M. Kołaczkowski, E. Carvajal, E. Balzi, A. Goffeau
- BiologyJournal of Molecular Microbiology and…
- 1 April 2001
Three plasma membrane multidrug exporters are studied in detail and the substrate specificity of Pdr5p, Snq2p and Yor1p are largely, but not totally, overlapping as shown by screening the growth inhibition by 349 toxic compounds of combinatorial deletants of these three ABC genes.
In vivo characterization of the drug resistance profile of the major ABC transporters and other components of the yeast pleiotropic drug resistance network.
- M. Kołaczkowski, A. Kolaczowska, J. Luczyński, S. Witek, A. Goffeau
- BiologyMicrobial Drug Resistance
Identification of compounds showing Pdr1p, Pdr3p-dependent, but Pdr5p-, Snq2p-, and Yor1p-independent toxicity suggests the involvement of new drug resistance genes and is a first step toward their identification.
Solubilization and characterization of the overexpressed PDR5 multidrug resistance nucleotide triphosphatase of yeast.
Compensatory activation of the multidrug transporters Pdr5p, Snq2p, and Yor1p by Pdr1p in Saccharomyces cerevisiae
Drug resistance mechanisms and their regulation in non-albicans Candida species.
Findings concerning underlying mechanisms associated with elevated drug resistance suggest a link to cell wall and plasma membrane metabolism in non-albicans Candida species.
Functional dissection of Pdr1p, a regulator of multidrug resistance in Saccharomyces cerevisiae
- A. Kołaczkowska, M. Kołaczkowski, A. Delahodde, A. Goffeau
- BiologyMolecular Genetics and Genomics
- 20 February 2002
A mini-Pdr1p consisting of only the DNA-binding and the activation domains strongly up-regulates the expression of the major target genes PDR5, SNQ2 and YOR1, resulting in enhanced multidrug resistance.
Novel multi-target-directed ligands for Alzheimer's disease: Combining cholinesterase inhibitors and 5-HT6 receptor antagonists. Design, synthesis and biological evaluation.
Differential Regulation of Ceramide Synthase Components LAC1 and LAG1 in Saccharomyces cerevisiae
- M. Kołaczkowski, A. Kołaczkowska, B. Gaigg, R. Schneiter, W. S. Moye-Rowley
- BiologyEukaryotic Cell
- 1 August 2004
Detailed analyses of the LAC1 promoter demonstrated that transcription of this gene is inhibited by the presence of the transcription factor Cbf1p and the anaerobic repressor Rox1p, demonstrating extensive coordinate control of sphingolipid biosynthesis and multidrug resistance in yeast.
Fungal ABC transporter-associated activity of isoflavonoids from the root extract of Dalea formosa.
The results demonstrate that multiple crude extract compounds are differentially affected by efflux-mediated resistance and are collectively responsible for the observed bioactivity.